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What is the definition of a chemically-defined medium?  

...can contain recombinant proteins. Due to current developments in the cell and gene therapy industry, there has been an increased focus on contaminants that may cause unfavorable reactions with patient material.  ...
Missing: stable linesGuideline

How can I run a standard curve for ToxiLight™ Non-Destructive Cytotoxicity BioAssay?

...a sample of their untreated cells at 56°C for 1h.Measuring this and then comparing it with the RLUs produced from their samples will enable them to determine percent cytoxicity if that is what they require....
Missing: stable linesGuideline

What is the effect of pH on the Luciferase-based BioAssays (ViaLight™ Plus, MycoAlert®, ToxiLight®) Lonza is offering?

...but obviously if the pH is very low it will not be able to do this adequately.ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay should be the least affected by this as it works by acidifying the sample and then has a very strong buffering system...
Missing: stable linesGuideline

Why does Lonza suggest multiple concentrations of Hydrocortisone-21-hemisuccinate and ß-Glycerophosphate when differentiating Normal Human Osteoblasts (NHOst)?

...of hydrocortisone-21-hemisuccinate and/or ß-glycerophosphate. While high concentrations of these reagents may cause greater differentiation in some lots, the same concentrations may cause cells to slough and die in other lots. ???????As such, Lonza suggests trying varying...
Missing: stable linesGuideline

Is there any problem using the Vialight™ Plus Kit due to the presence of immobilized antibodies on the bottom of the microplate?

...antibody to be tested and detect the ATP by treating the wells as if they contained cells following the protocol contained in the kit. If you use the ViaLight HS kit add 100ul of NRR to each well and wait 5 min before adding 20ul of AMR. The difference...
Missing: stable linesGuideline

In my Nucleofection™ Experiment I see lower expression with my IRES construct in comparison with you pmax-GFP control. Do you have any information about the different expression profiles?

...the Nucleofector™ Technology" The data generated by our Research department show the different expression profiles of 3 vectors in HL-60 and HUVEC cells. You can see that our pmaxGFP cloned into three different vector sites show a different expression level....
Missing: stable linesGuideline

What is the difference between the EBM™ and EBM™-2 Basal Media? Can the EBM™-2 Basal Media be substituted with the EBM™ phenol red free Basal Media?

...The two basal media can be used interchangeably so long as the appropriate SingleQuot™ Kit supplements are added depending on the specific cell type being cultured. Additionally, both the EBM™ and EBM™-2 Basal Media can be substituted with the EBM™ Phenol...
Missing: stable linesGuideline

Are there positive controls for the MycoAlert® Assay?

...are designed for use with the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit for the detection of mycoplasma contaminations in cell cultures. The MycoAlert® Assay Control Set may be used to ensure that the operator and all...
Missing: stable linesGuideline

I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?

We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual...
Missing: stable linesGuideline

What are the best time points for analysis of my siRNA experiments?

As the stability and half-life of various mRNAs and their protein products varies, it is important to empirically determine the best time points for assessing target knockdown. For example, it has been documented that in mammalian cells, mRNA...
Missing: stable linesGuideline
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