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Can I use larger cuvettes for my Nucleofection® Reaction? Can I use the cuvettes more than once?

No. The electrical parameters provided by the Nucleofector® System are optimized for the cuvettes contained in the Nucleofector® Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher cell death and lower...
Missing: stable linesGuideline

Do bacteria lead to false positive MycoAlert® Results?

...is characterized by rapid appearance of turbidity in cell culture media. One of the dangers of mycoplasma infection is that they do not produce turbidity, hence the need for a test such as the MycoAlert® Assay....
Missing: stable linesGuideline

What is a Clonetics™ or Poietics™ Basal Medium?

The Clonetics™ and Poietics™ Basal Media are primarily composed of trace metals, amino acids, vitamins, salts, and water. The basal medium contains no growth factors or antibiotics and is not sufficient to support cell attachment and growth...
Missing: stable linesGuideline

I’m looking for the plasmid map, particularly the sequence of the pmax-GFP plasmid provided with your nucleofection kits. Could you provide me this information?

...sequence. The copGFP is not suitable for long term expression, in some specific cell types like neurons  it builds aggregates which might be toxic for some cells. The further development of the copGFP is the turbo GFP.Lonza has discontinued...
Missing: stable linesGuideline

Which reporter gene should I choose for my reporter gene experiment?

In general, all reporter genes can be used.For some applications (e.g. reporter gene experiments in various primary cells, experiments with late analysis time point due to starving and /or stimulation) a reporter gene with a longer half life than...
Missing: stable linesGuideline

Where are Lonza’s aortic adventitial fibroblasts (AoAF) isolated from?

...muscle cells, and the tunica adventitia is the outermost layer where the aortic adventitial fibroblasts (AoAF) are isolated from. Aortic adventitial fibroblasts (AoAF) are isolated from either ascending or descending aorta....
Missing: stable linesGuideline

Why are Lonza’s adult melanocytes cultured with Endothelin-3 (ET-3) while the neonatal melanocytes are not cultured with this reagent?

Adult melanocytes typically have a very slow doubling time, especially in comparison to neonatal melanocytes. Endothelin-3 (ET-3) is added to adult melanocytes only to improve the doubling time and, in the process, help to maintain the cells...
Missing: stable linesGuideline

What does the message "weak" mean on the display of the Nucleofector® IIN, IIS or 2b after Nucleofection®?

...expected. So it is worthwhile to analyze the sample, keeping in mind that the transfection efficiency might be a bit lower. We don't recommend applying the pulse to the same cuvette again, as this will most likely result in high mortality of your cells....
Missing: stable linesGuideline

How to prepare a BulletKit™ Medium?

...media can be stored at 4°C for up to 30 days. Exceptions include the ALI differentiation media, mouse and rat neuronal media, and preadipocyte media. Please see the specific cell instructions for more information....
Missing: stable linesGuideline

What is the definition of a chemically-defined medium?  

...can contain recombinant proteins. Due to current developments in the cell and gene therapy industry, there has been an increased focus on contaminants that may cause unfavorable reactions with patient material.  ...
Missing: stable linesGuideline
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