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What cells make up the population of cells in Lonza's bone marrow stromal cells?

The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.
Missing: stable linesGuideline

What cells make up the mononuclear cell population?

Mononuclear cells are any blood cells with a rounded nucleus including lymphocytes (T-cells, B-cells, and Natural Killer [NK] Cells), monocytes, and dendritic cells.
Missing: stable linesGuideline

Where are Lonza’s RPTEC cells CC-2553 isolated from? Is Lonza certain that these cells are truly RPTEC?

Lonza’s Renal Proximal Tubule Epithelial Cells (RPTEC) are isolated from the cortex of the kidney. A characteristic of kidney cortex epithelial isolation results in a population consisting of approximately 50%- 70% RPTEC. Lonza uses a proprietary...
Missing: stable linesGuideline

Can I use the pmaxGFP™ control plasmid with insect cells such as S2 cells or SF9 cells?

The pmaxGFP™ Vector provided in our Nucleofector® Kits is not expressed in insect cells. We strongly recommend an insect expression vector encoding a fluorescent protein or lacZ reporter as a positive control for your experiments [e.g. Novagen™’s...
Missing: stable linesGuideline

Your instructions for culturing of Clonetics™ Cells do not mention centrifuging the cells upon thawing. Won't the DMSO kill the cells?

We have seen on multiple occasions that the effects of centrifugation can be significantly more damaging than the effects of residual DMSO on primary cells. As such, unless specifically noted in the recommended culture protocol, instead...
Missing: stable linesGuideline

I am using the acute leukemia HL-60 cell line for CAR-T cells experiments. Do you have a transfection protocol for genome editing?

The HL-60 cells are often used as a model for in vitro research for treatment of AML (acute myeloid leukemia) in combination with modified primary human CAR- T cells (T cells expressing chimeric antigen receptors).Transfection protocols can be found...
Missing: stable linesGuideline

What is the percentage of CD34+ cells in the bone marrow mononuclear cell population?

CD34+ cells make up about 2% of the population in bone marrow mononuclear cells.
Missing: stable linesGuideline

With regards to mesenchymal stem cells, what are "Dexter cultures"?

Dexter culture refers to the method of isolating stem cells proposed by Dexter et al in their 1977 paper: Dexter TM, Allen TD, Lajtha LG. Conditions controlling the proliferation of haemopoietic stem cells in vitro. JCell Physiol 1977; 91: 335–344...
Missing: stable linesGuideline

Where are Lonza’s PrSMC cells isolated from?

Lonza’s Prostate Smooth Muscle Cells (PrSMC) are isolated from the stroma and the vasculature of the prostate. Unfortunately, there is no way to distinguish between smooth muscle cells from the stroma and smooth muscle cells from the vasculature...
Missing: stable linesGuideline

What are your recommendations for minimum and maximum cell numbers for Nucleofection™ ?

The recommended cell number will vary depending on which Optimized protocol is being used. In general, using less than 2x10exp5cells per reaction causes a major increase in cell mortality. For some cell lines we have tried cell numbers up...
Missing: stable linesGuideline
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