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Do you have recommendations for the type of samples used for the
MycoAlert
® Mycoplasma Detection Kit and the
MycoAlert
® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?
The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...
Missing:
plate
Is it possible to pre-
plate
substrates when using the 96-well Shuttle® for Nucleofection®?
Yes, pre-
plating
of substrates like siRNA and subsequent storage at -20°C is possible.
Missing:
mycoalert
PyroTec Pro: How many
plate
s can be processed with the instrument?
The system can accommodate up to two independent microplates on a single run of the instrument.
Missing:
mycoalert
Can I
plate
cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?
Lonza's recommended
plating
density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage...
Missing:
mycoalert
How long is the
MycoAlert
® Mycoplasma Detection Assay signal stable?
Please follow the protocol exactly as written – add reagent, wait 5 min and read, add substrate, wait 10 minutes and read again.
Missing:
plate
How much sample do you need for accurate results with
MycoAlert
® and
MycoAlert
® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?
The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing:
plate
What temperature should the ToxiLight™, ViaLight™ and
MycoAlert
™ Assays be run at?
The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.
Missing:
plate
I tested my cell culture supernatant negative for mycoplasma contamination using the
MycoAlert
® Mycoplasma Detection Kit or the
MycoAlert
® PLUS Mycoplasma Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
It is possible that the PCR is picking up dead mycoplasma whereas the MycoAlert® and
MycoAlert
® Plus Assay will pick up only viable mycoplasma. It is possible of course that there is contamination in the PCR giving a false positive.
Missing:
plate
What
plate
format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?
The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for
plating
cells into a 96-well
plate
.
Missing:
mycoalert
Do the Clonetics™ Rat and Mouse Astrocytes require an extracellular matrix for plating?
No, they simply need to be seeded onto tissue culture treated plastic. For best performance, we recommend to use positively charged plasticware
Missing:
mycoalert
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