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Can I freeze the 96-well Nucleocuvette®
Plate
s?
Yes. Freezing of the
plates
at -20°C does not alter their properties.
Missing:
mycoalert
Is it possible to pre-
plate
substrates when using the 96-well Shuttle® for Nucleofection®?
Yes, pre-
plating
of substrates like siRNA and subsequent storage at -20°C is possible.
Missing:
mycoalert
PyroTec Pro: How many
plate
s can be processed with the instrument?
The system can accommodate up to two independent microplates on a single run of the instrument.
Missing:
mycoalert
Can I
plate
cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?
Lonza's recommended
plating
density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage...
Missing:
mycoalert
How long is the
MycoAlert
® Mycoplasma Detection Assay signal stable?
Please follow the protocol exactly as written – add reagent, wait 5 min and read, add substrate, wait 10 minutes and read again.
Missing:
plate
How much sample do you need for accurate results with
MycoAlert
® and
MycoAlert
® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?
The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing:
plate
I tested my cell culture supernatant negative for mycoplasma contamination using the
MycoAlert
® Mycoplasma Detection Kit or the
MycoAlert
® PLUS Mycoplasma Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
It is possible that the PCR is picking up dead mycoplasma whereas the MycoAlert® and
MycoAlert
® Plus Assay will pick up only viable mycoplasma. It is possible of course that there is contamination in the PCR giving a false positive.
Missing:
plate
What temperature should the ToxiLight™, ViaLight™ and
MycoAlert
™ Assays be run at?
The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.
Missing:
plate
Can I also use the Lucetta® 2 Luminometer for other assays besides the
MycoAlert
® Mycoplasma Detection Assay?
Yes. In addition to the tailor-made
MycoAlert
® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based cell proliferation or cytotoxicity assays, or Luciferase reporter gene assays.
Missing:
plate
What
plate
format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?
The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for
plating
cells into a 96-well
plate
.
Missing:
mycoalert
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