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Can I freeze the 96-well Nucleocuvette®
Plate
s?
Yes. Freezing of the
plates
at -20°C does not alter their properties.
Missing:
mycoalert
Is it possible to pre-
plate
substrates when using the 96-well Shuttle® for Nucleofection®?
Yes, pre-
plating
of substrates like siRNA and subsequent storage at -20°C is possible.
Missing:
mycoalert
PyroTec Pro: How many
plate
s can be processed with the instrument?
The system can accommodate up to two independent microplates on a single run of the instrument.
Missing:
mycoalert
Can I
plate
cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?
Lonza's recommended
plating
density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage...
Missing:
mycoalert
How long is the
MycoAlert
® Mycoplasma Detection Assay signal stable?
Please follow the protocol exactly as written – add reagent, wait 5 min and read, add substrate, wait 10 minutes and read again.
Missing:
plate
How much sample do you need for accurate results with
MycoAlert
® and
MycoAlert
® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?
The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing:
plate
What temperature should the ToxiLight™, ViaLight™ and
MycoAlert
™ Assays be run at?
The optimal working temperature for all reagents is 22°C.If reagents have been refrigerated always allow time for them to reach room temperature before use.
Missing:
plate
I tested my cell culture supernatant negative for mycoplasma contamination using the
MycoAlert
® Mycoplasma Detection Kit or the
MycoAlert
® PLUS Mycoplasma Detection Kit. But when I tested the same cells using a PCR-based Assay, I obtained a positive result.
It is possible that the PCR is picking up dead mycoplasma whereas the MycoAlert® and
MycoAlert
® Plus Assay will pick up only viable mycoplasma. It is possible of course that there is contamination in the PCR giving a false positive.
Missing:
plate
Can I also use the Lucetta® 2 Luminometer for other assays besides the
MycoAlert
® Mycoplasma Detection Assay?
Yes. In addition to the tailor-made
MycoAlert
® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based cell proliferation or cytotoxicity assays, or Luciferase reporter gene assays.
Missing:
plate
When performing the
MycoAlert
® Mycoplasma Detection Assay, why is it necessary to spin out the cells?
It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background and...
Missing:
plate
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