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Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight™?

...of the enzyme adenylate kinase released from cells undergoing necrosis, this will obviously be denatured by the SDS. ToxiLight™ is a bioluminescent based assay which uses the firefly enzyme luciferase, the luciferase will also be denatured by the SDS. You...
Missing: stable lines

What is the amount of siRNA needed per Nucleofection® Reaction?

...For Nucleofection®, the optimal siRNA concentration can range from lower than 2 nM up to 2 µM, depending on multiple factors such as the cell type, and the half-life of the mRNA and/or protein of the gene target. To determine the optimal concentration for your cell...
Missing: stable lines

What factors interfere with the ViaLight™ Plus Assay?

...in cell culture).  Fetal Calf Serum also quenches but levels of FCS below 10% have no significant effect on assay sensitivity.  Concentrations above 10% may reduce the sensitivity of the assay.  When investigating dose-response relationships...
Missing: stable lines

Do your Clonetics® Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.
Missing: stable lines

I’m looking for the plasmid map, particularly the sequence of the pmax-GFP plasmid provided with your nucleofection kits. Could you provide me this information?

...sequence. The copGFP is not suitable for long term expression, in some specific cell types like neurons  it builds aggregates which might be toxic for some cells. The further development of the copGFP is the turbo GFP.Lonza has discontinued...
Missing: stable lines

Are rat and mouse hepatocytes transfected by Nucleofection® still functional?

Yes, transfected mouse hepatocytes show albumin synthesis rates comparable to those of untransfected cells. Moreover the morphology and polarization of rat hepatocytes is not affected by Nucleofection®.
Missing: stable lines

What is the lowest amount of Human Hepatocytes that can be used with the 96-well Shuttle® System or the 4D-Nucleofector® X-Unit?

Human hepatocytes do not like to be cultured and transfected at low densities. Therefore, using less than 100,000 cells per Nucleofection® is not recommended.
Missing: stable lines

Do I need to heat inactivate the FBS contained in the SingleQuots™ Kit?

No, we do not recommend heat inactivating the FBS provided in the SingleQuots™ Kit. Heat inactivating the serum can alter cell performance and denature essential components in the serum.
Missing: stable lines

For hepatocytes, is plating density a concern post Nucleofection®?

Yes, the optimized protocols for the standard Nucleofector® recommend the use of 6 well plates. Plating into a 12-well plate will pose a problem as less cells will attach at high densities.
Missing: stable lines

What is the advantage of using conductive polymer as electrode material for the 4D-Nucleofector® X-Unit and 96-well Unit?

With conductive polymer electrodes the release of metal ions into the cell suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit.
Missing: stable lines
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