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For hepatocytes, is plating density a concern post Nucleofection®?
Yes, the optimized protocols for the standard Nucleofector® recommend the use of 6 well plates. Plating into a 12-well plate will pose a problem as less
cells
will attach at high densities.
Missing:
stable
lines
What is the advantage of using conductive polymer as electrode material for the 4D-Nucleofector® X-Unit and 96-well Unit?
With conductive polymer electrodes the release of metal ions into the
cell
suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?
Missing:
stable
lines
Do you recommend positive selection or depletion for the purification of Human monocytes for Nucleofection®?
We only recommend the depletion method. For Example, the RosetteSep™ Isolation Kit for human monocytes [Stem
Cell
Technologies, Cat. No 15028]. The advantage of depletion is that the monocytes are left untouched by antibodies during the process.
Missing:
stable
lines
Can I also use the Lucetta® 2 Luminometer for other assays besides the MycoAlert® Mycoplasma Detection Assay?
Yes. In addition to the tailor-made MycoAlert® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based
cell
proliferation or cytotoxicity assays, or Luciferase reporter gene assays.
Missing:
stable
lines
Where are Lonza’s lung fibroblasts (NHLF and DHLF) isolated from?
...separation and growth in a
cell
specific growth medium further purify the population....
Missing:
stable
lines
Do we know the severity of the asthma or the genotype of the cystic fibrosis donors?
Generally, information on the severity of asthma is not available. For our diseased
cells
from donors with cystic fibrosis, we usually have information on the genotype of the donor. Please contact our scientific support department for further...
Missing:
stable
lines
How do I promote keratinocyte terminal differentiation towards corneocytes?
Keratinocyte differentiation can be induced by increasing the concentration of calcium and/or by adding serum to the media.
Cells
should be initially plated using the standard culture media and conditions and allowed ample time to recover from...
Missing:
stable
lines
Do your ProCHO™ Media contain hypoxanthine and thymidine?
All of our ProCHO™ Media, doe not contain Thymidine and Hypoxanthine. So if the customer has a CHO Clone which is dhfr-negativ, he will need to add Thymidine and Hypoxanthine or otherwise the
cells
will not grow. If the customer has a CHO clone...
Missing:
stable
lines
What is the best incubation time for transient gene expression in Nucleofection® Experiments?
The optimal incubation time ranges from 2 to 6 days depending on various factors like the stability of the protein in the medium, potential toxicity of the protein,
cell
density and stability of the plasmid in the nucleus. To check the timepoint...
Missing:
stable
lines
Can I use larger cuvettes for my Nucleofection® Reaction? Can I use the cuvettes more than once?
No. The electrical parameters provided by the Nucleofector® System are optimized for the cuvettes contained in the Nucleofector® Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher
cell
death and lower...
Missing:
stable
lines
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