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Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...
Missing: stable linesGuideline

Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?

The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95...
Missing: stable linesGuideline

Why do I need endotoxin-free materials to conduct the PyroCell® Monocyte Activation Test (MAT)?

...should be prepared with materials that do not contain assayable levels of endotoxin which means that endotoxin contents are below the detection limit of the MAT assay.In addition, the cell culture step must be prepared aseptically using sterile...
Missing: stable linesGuideline

Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight™?

...of the enzyme adenylate kinase released from cells undergoing necrosis, this will obviously be denatured by the SDS. ToxiLight™ is a bioluminescent based assay which uses the firefly enzyme luciferase, the luciferase will also be denatured by the SDS. You...
Missing: stable linesGuideline

What is the amount of siRNA needed per Nucleofection® Reaction?

...For Nucleofection®, the optimal siRNA concentration can range from lower than 2 nM up to 2 µM, depending on multiple factors such as the cell type, and the half-life of the mRNA and/or protein of the gene target. To determine the optimal concentration for your cell...
Missing: stable linesGuideline

Do your Clonetics™ Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.
Missing: stable linesGuideline

What is the difference between the EGM™ and EGM™-2 growth media? What is the difference between the EGM™-MV and EGM™-2MV growth media?

...and epidermal growth factor (hEGF).Cells tend to proliferate better in the EGM™-2 and EGM™-2MV growth media versions, however some customers have indicated that the original EGM™ and EGM™MV growth media works better for VEGF, FGF and angiogenesis research...
Missing: stable linesGuideline

What factors interfere with the ViaLight™ Plus Assay?

...in cell culture).  Fetal Calf Serum also quenches but levels of FCS below 10% have no significant effect on assay sensitivity.  Concentrations above 10% may reduce the sensitivity of the assay.  When investigating dose-response relationships...
Missing: stable linesGuideline

Are rat and mouse hepatocytes transfected by Nucleofection® still functional?

Yes, transfected mouse hepatocytes show albumin synthesis rates comparable to those of untransfected cells. Moreover the morphology and polarization of rat hepatocytes is not affected by Nucleofection®.
Missing: stable linesGuideline

What is the lowest amount of Human Hepatocytes that can be used with the 96-well Shuttle® System or the 4D-Nucleofector® X-Unit?

Human hepatocytes do not like to be cultured and transfected at low densities. Therefore, using less than 100,000 cells per Nucleofection® is not recommended.
Missing: stable linesGuideline
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