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I’m looking for the plasmid map, particularly the sequence of the pmax-GFP plasmid provided with your nucleofection kits. Could you provide me this information?
...sequence. The copGFP is not suitable for long term expression, in some specific
cell
types like neurons it builds aggregates which might be toxic for some
cells
. The further development of the copGFP is the turbo GFP.Lonza has discontinued...
Missing:
stable
lines
Do your Clonetics™ Rat and Mouse Astrocytes proliferate?
Yes. The
cells
do expand in culture.
Missing:
stable
lines
Are rat and mouse hepatocytes transfected by Nucleofection® still functional?
Yes, transfected mouse hepatocytes show albumin synthesis rates comparable to those of untransfected
cells
. Moreover the morphology and polarization of rat hepatocytes is not affected by Nucleofection®.
Missing:
stable
lines
What is the lowest amount of Human Hepatocytes that can be used with the 96-well Shuttle® System or the 4D-Nucleofector® X-Unit?
Human hepatocytes do not like to be cultured and transfected at low densities. Therefore, using less than 100,000
cells
per Nucleofection® is not recommended.
Missing:
stable
lines
Do I need to heat inactivate the FBS contained in the SingleQuots™ Kit?
No, we do not recommend heat inactivating the FBS provided in the SingleQuots™ Kit. Heat inactivating the serum can alter
cell
performance and denature essential components in the serum.
Missing:
stable
lines
For hepatocytes, is plating density a concern post Nucleofection®?
Yes, the optimized protocols for the standard Nucleofector® recommend the use of 6 well plates. Plating into a 12-well plate will pose a problem as less
cells
will attach at high densities.
Missing:
stable
lines
What is the advantage of using conductive polymer as electrode material for the 4D-Nucleofector® X-Unit and 96-well Unit?
With conductive polymer electrodes the release of metal ions into the
cell
suspension is avoided. Conductive polymer electrode Nucloecuvette® Vessels are used in the 4D-Nucleofector® X-Unit and 96-well Unit?
Missing:
stable
lines
Do you recommend positive selection or depletion for the purification of Human monocytes for Nucleofection®?
We only recommend the depletion method. For Example, the RosetteSep™ Isolation Kit for human monocytes [Stem
Cell
Technologies, Cat. No 15028]. The advantage of depletion is that the monocytes are left untouched by antibodies during the process.
Missing:
stable
lines
Can I also use the Lucetta® 2 Luminometer for other assays besides the MycoAlert® Mycoplasma Detection Assay?
Yes. In addition to the tailor-made MycoAlert® Mode it also has a "Single Read" mode which is suited for unprocessed luminescence readings, e.g. ATP-based
cell
proliferation or cytotoxicity assays, or Luciferase reporter gene assays.
Missing:
stable
lines
Where are Lonza’s lung fibroblasts (NHLF and DHLF) isolated from?
...separation and growth in a
cell
specific growth medium further purify the population....
Missing:
stable
lines
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