Data Type
Cell Information
(526)
Citations
(4826)
FAQ
(320)
Culture Media
(46)
Category
Primary Cells and Media
(160)
Transfection
(107)
Bioassay
(32)
Mycoplasma Detection and Prevention
(16)
Endotoxin and Pyrogen Testing
(14)
Laboratory Instrumentation
(14)
Serum-free and Speciality Media
(14)
Cell Lines and Primary Cancer Cells
(7)
Classical Media and Reagents
(6)
3D Cell Culture
(5)
Cell Services
(3)
Bioprocess Containers
(2)
Live Cell Imaging
(1)
+ Show All
Research Area
Basic Research
(101)
Cancer Research/Cell Biology
(68)
Immunotherapy / Hematology
(54)
Gene Expression
(39)
Stem Cells
(34)
Respiratory Research
(26)
Cardiovascular
(21)
Regenerative medicine
(18)
Uncategorized
(18)
Neurobiology
(17)
Toxicology
(16)
Dermatology/Tissue Engineering
(8)
Drug Discovery
(6)
Molecular Biology
(5)
Endotoxin Testing
(3)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
320 results sorted by
relevance
alphabetical
newest first
oldest first
I transfected neurons using the 4D-Nucleofector® Y-Unit. After Nucleofection®, there were precipitates in my culture. What can I do?
Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing
cell
adherence, but add medium quickly to avoid
cells
...
Missing:
stable
lines
How does TheraPEAK 293-GT® Media System compare to other AAV production media on the market?
Download our application note or product sheet to see
cell
growth,
cell
viability, viral titer and viral capsid data for TheraPEAK 293-GT® Media System as compared to the industry leading complete production kit and the industry leading media...
Missing:
stable
lines
What is the shelf life of the Pyro
Cell
® MAT System Kits?
Except for the pMAT
cells
, the shelf life of each component is indicated on the vial label or the kit box label. The pMAT
cells
can be used for up to 6 months as of receiving.Pyro
Cell
® MAT System Kits component should be used beyond its expiry...
Missing:
stable
lines
What media can the CD14+ Monocytes be cultured in to maintain the monocyte phenotype?
CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The
cells
will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence...
Missing:
stable
lines
Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?
Lonza R&D has found some NHOst
cell
strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the
cells
will lift off dish, curl up and die. As such, Lonza recommends...
Missing:
stable
lines
What is the difference between the EGM™ and EGM™-2 growth media? What is the difference between the EGM™-MV and EGM™-2MV growth media?
...and epidermal growth factor (hEGF).
Cells
tend to proliferate better in the EGM™-2 and EGM™-2MV growth media versions, however some customers have indicated that the original EGM™ and EGM™MV growth media works better for VEGF, FGF and angiogenesis research...
Missing:
stable
lines
When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?
...will result in the
cells
losing their differentiated phenotype until the culture is almost 100% de-differentiated and no longer express collagen. For optimal re-differentiation,
cells
should be expanded as few times as necessary prior to re-differentiation....
Missing:
stable
lines
Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?
Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated
cells
which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...
Missing:
stable
lines
Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?
The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in
cell
culture). 95...
Missing:
stable
lines
Why do I need endotoxin-free materials to conduct the Pyro
Cell
® Monocyte Activation Test (MAT)?
...should be prepared with materials that do not contain assayable levels of endotoxin which means that endotoxin contents are below the detection limit of the MAT assay.In addition, the
cell
culture step must be prepared aseptically using sterile...
Missing:
stable
lines
PREVIOUS
PAGE
24
PAGE
25
PAGE
26
PAGE
27
PAGE
28
PAGE
29
PAGE
30
PAGE
31
PAGE
32
NEXT