Data Type

Category

+ Show All

Research Area

+ Show All
315 results sorted by

What is the amount of DNA I can use in my Nucleofection® Experiment?

...of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution.Furthermore, some cells might be sensitive to larger amounts of DNA...
Missing: stable lines

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

...will result in the cells losing their differentiated phenotype until the culture is almost 100% de-differentiated and no longer express collagen. For optimal re-differentiation, cells should be expanded as few times as necessary prior to re-differentiation....
Missing: stable lines

Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...
Missing: stable lines

Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?

The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95...
Missing: stable lines

Why do I need endotoxin-free materials to conduct the PyroCell® Monocyte Activation Test (MAT)?

...should be prepared with materials that do not contain assayable levels of endotoxin which means that endotoxin contents are below the detection limit of the MAT assay.In addition, the cell culture step must be prepared aseptically using sterile...
Missing: stable lines

Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight™?

...of the enzyme adenylate kinase released from cells undergoing necrosis, this will obviously be denatured by the SDS. ToxiLight™ is a bioluminescent based assay which uses the firefly enzyme luciferase, the luciferase will also be denatured by the SDS. You...
Missing: stable lines

What is the amount of siRNA needed per Nucleofection® Reaction?

...For Nucleofection®, the optimal siRNA concentration can range from lower than 2 nM up to 2 µM, depending on multiple factors such as the cell type, and the half-life of the mRNA and/or protein of the gene target. To determine the optimal concentration for your cell...
Missing: stable lines

What is the difference between the EGM™ and EGM™-2 growth media? What is the difference between the EGM™-MV and EGM™-2MV growth media?

...and epidermal growth factor (hEGF).Cells tend to proliferate better in the EGM™-2 and EGM™-2MV growth media versions, however some customers have indicated that the original EGM™ and EGM™MV growth media works better for VEGF, FGF and angiogenesis research...
Missing: stable lines

What factors interfere with the ViaLight™ Plus Assay?

...in cell culture).  Fetal Calf Serum also quenches but levels of FCS below 10% have no significant effect on assay sensitivity.  Concentrations above 10% may reduce the sensitivity of the assay.  When investigating dose-response relationships...
Missing: stable lines

Do your Clonetics™ Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.
Missing: stable lines
PAGE 28

Privacy | Legal | About Lonza

© Lonza. All rights reserved.