Data Type

Category

+ Show All

Research Area

+ Show All
319 results sorted by

How many culture inserts/transwells can be supported with one B-ALI™ Media Kit?

One ampoule of Normal Human Bronchial Epithelial Cells (NHBE) together with one B-ALI™ Media Kit is sufficient to establish a 24-well plate culture for at least one month. Under recommended culture conditions, the cells typically develop beating...
Missing: stable lines

What media can the CD14+ Monocytes be cultured in to maintain the monocyte phenotype?

CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The cells will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence...
Missing: stable lines

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...
Missing: stable lines

What is the amount of DNA I can use in my Nucleofection® Experiment?

...of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution.Furthermore, some cells might be sensitive to larger amounts of DNA...
Missing: stable lines

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

...will result in the cells losing their differentiated phenotype until the culture is almost 100% de-differentiated and no longer express collagen. For optimal re-differentiation, cells should be expanded as few times as necessary prior to re-differentiation....
Missing: stable lines

Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...
Missing: stable lines

What is the difference between the EGM™ and EGM™-2 growth media? What is the difference between the EGM™-MV and EGM™-2MV growth media?

...and epidermal growth factor (hEGF).Cells tend to proliferate better in the EGM™-2 and EGM™-2MV growth media versions, however some customers have indicated that the original EGM™ and EGM™MV growth media works better for VEGF, FGF and angiogenesis research...
Missing: stable lines

Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?

The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95...
Missing: stable lines

Why do I need endotoxin-free materials to conduct the PyroCell® Monocyte Activation Test (MAT)?

...should be prepared with materials that do not contain assayable levels of endotoxin which means that endotoxin contents are below the detection limit of the MAT assay.In addition, the cell culture step must be prepared aseptically using sterile...
Missing: stable lines

Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight™?

...of the enzyme adenylate kinase released from cells undergoing necrosis, this will obviously be denatured by the SDS. ToxiLight™ is a bioluminescent based assay which uses the firefly enzyme luciferase, the luciferase will also be denatured by the SDS. You...
Missing: stable lines
PAGE 28

Privacy | Legal | About Lonza

© Lonza. All rights reserved.