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Can I use the Nucleofector® 2b Solutions and Optimized Protocols with the 4D-Nucleofector® X-Unit or the 96-well Shuttle® System?

No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...
Missing: stable linesGuideline

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

...will result in the cells losing their differentiated phenotype until the culture is almost 100% de-differentiated and no longer express collagen. For optimal re-differentiation, cells should be expanded as few times as necessary prior to re-differentiation....
Missing: stable linesGuideline

Why must Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) be re-differentiated to express collagen?

Lonza’s Normal Human Articular Chondrocytes (NHAC-kn) are differentiated cells which express collagen when they are initially isolated, however, they lose their differentiated phenotype after serial expansion in monolayer culture so that by the 2nd...
Missing: stable linesGuideline

Does the MycoAlert® Assay recognize the whole spectrum of mycoplasma?

The mycoplasma specific metabolism detected by the MycoAlert® Assay is present in all members of the mollicute family (Mycoplasma, Acholeplasma, Entomoplasma and Spiroplasma) except Ureaplasma (which are no usual suspects in cell culture). 95...
Missing: stable linesGuideline

Can I use SDS as a detergent for a positive control if I do cytotoxicity assay with ToxiLight™?

...of the enzyme adenylate kinase released from cells undergoing necrosis, this will obviously be denatured by the SDS. ToxiLight™ is a bioluminescent based assay which uses the firefly enzyme luciferase, the luciferase will also be denatured by the SDS. You...
Missing: stable linesGuideline

What is the amount of siRNA needed per Nucleofection® Reaction?

...For Nucleofection®, the optimal siRNA concentration can range from lower than 2 nM up to 2 µM, depending on multiple factors such as the cell type, and the half-life of the mRNA and/or protein of the gene target. To determine the optimal concentration for your cell...
Missing: stable linesGuideline

What is the difference between the EGM™ and EGM™-2 growth media? What is the difference between the EGM™-MV and EGM™-2MV growth media?

...and epidermal growth factor (hEGF).Cells tend to proliferate better in the EGM™-2 and EGM™-2MV growth media versions, however some customers have indicated that the original EGM™ and EGM™MV growth media works better for VEGF, FGF and angiogenesis research...
Missing: stable linesGuideline

What factors interfere with the ViaLight™ Plus Assay?

...in cell culture).  Fetal Calf Serum also quenches but levels of FCS below 10% have no significant effect on assay sensitivity.  Concentrations above 10% may reduce the sensitivity of the assay.  When investigating dose-response relationships...
Missing: stable linesGuideline

I’m looking for the plasmid map, particularly the sequence of the pmax-GFP plasmid provided with your nucleofection kits. Could you provide me this information?

...sequence. The copGFP is not suitable for long term expression, in some specific cell types like neurons  it builds aggregates which might be toxic for some cells. The further development of the copGFP is the turbo GFP.Lonza has discontinued...
Missing: stable linesGuideline

Do your Clonetics™ Rat and Mouse Astrocytes proliferate?

Yes. The cells do expand in culture.
Missing: stable linesGuideline
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