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I transfected neurons using the 4D-Nucleofector® Y-Unit. After Nucleofection®, there were precipitates in my culture. What can I do?

Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells...
Missing: stable linesGuideline

What are mycoplasmas?

...and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii).Mycoplasmas are widespread...
Missing: stable linesGuideline

What is the shelf life of the PyroCell® MAT System Kits?

Except for the pMAT cells, the shelf life of each component is indicated on the vial label or the kit box label. The pMAT cells can be used for up to 6 months as of receiving.PyroCell® MAT System Kits component should be used beyond its expiry...
Missing: stable linesGuideline

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
Missing: stable linesGuideline

What media can the CD14+ Monocytes be cultured in to maintain the monocyte phenotype?

CD14+ Monocytes can be cultured in IMDM + 10% FBS to help maintain the monocyte phenotype. The cells will slowly become adherent but it can take a week to get good adherence. M-CSF (10 ng/ml) will induce a little proliferation and increase adherence...
Missing: stable linesGuideline

Why does Lonza recommend two different concentrations of the B-Glycerophosphate in the Normal Human Osteoblasts (NHOst) differentiation protocol?

Lonza R&D has found some NHOst cell strains to be more sensitive to B-Glycerophosphate than others. While it is necessary to differentiate, at too high of concentrations the cells will lift off dish, curl up and die. As such, Lonza recommends...
Missing: stable linesGuideline

Why do plasmids which contain IRES sequences often have lower transfection efficiency?

...Since the downstream gene is usually GFP, the expression level of the GFP reporter will be lower than would be normally seen when compared to plasmids without an IRES-sequence. Thus the percentage of cells expressing detectable GFP fluorescence...
Missing: stable linesGuideline

Can I use the Nucleofector® 2b Solutions and Optimized Protocols with the 4D-Nucleofector® X-Unit or the 96-well Shuttle® System?

No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...
Missing: stable linesGuideline

What is the amount of DNA I can use in my Nucleofection® Experiment?

...of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution.Furthermore, some cells might be sensitive to larger amounts of DNA...
Missing: stable linesGuideline

When is the ideal time to study Lonza’s Human Knee Articular Chondrocytes (NHAC-kn) after re-differentiation?

...will result in the cells losing their differentiated phenotype until the culture is almost 100% de-differentiated and no longer express collagen. For optimal re-differentiation, cells should be expanded as few times as necessary prior to re-differentiation....
Missing: stable linesGuideline
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