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Can customers buy PBMCs from COVID-19 infected donors? 

At this time, we cannot offer any cells from active COVID-19 infected donors. We are not completely ruling this out as a possibility for future custom offerings, but at this time we cannot say if/when we may be able to offer this. What we can offer...
Missing: stable lines

Why do plasmids which contain IRES sequences often have lower transfection efficiency?

...Since the downstream gene is usually GFP, the expression level of the GFP reporter will be lower than would be normally seen when compared to plasmids without an IRES-sequence. Thus the percentage of cells expressing detectable GFP fluorescence...
Missing: stable lines

What are mycoplasmas?

...and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii).Mycoplasmas are widespread...
Missing: stable lines

What is the amount of DNA I can use in my Nucleofection® Experiment?

...of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution.Furthermore, some cells might be sensitive to larger amounts of DNA...
Missing: stable lines

How is the B-ALI™ Media System performance tested?

The media system is performance tested with our pre-screened NHBE cells and that system is guaranteed to form ciliated, mucous-producing cells after 3 weeks of culture.
Missing: stable lines

What is the amount of DNA needed per well when using the 96-well Shuttle® Device?

The amount depends on the cell type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some cells or constructs even 100 ng may be sufficient.
Missing: stable lines

What is the advantage of the Nucleofector® Technology over common electroporation (systems)?

High cell viability and high transfection efficiency with the Nucleofection® Technology are the most prominent features. Nucleofection®, i.e., the transfer of DNA into the nucleus and not only the cytosol, is essential when working...
Missing: stable lines

What is the best way to centrifuge Human Hepatocytes prior to Nucleofection™?

In order to avoid cell compaction in the pellet and difficulties in cell resuspension, we recommend using round bottom 2 ml vials or 50 ml BD Falcon™ tubes for all centrifugation steps prior to Nucleofection™.
Missing: stable lines

Can serum be used with Lonza's SAEC’s?

No. Serum will induce squamous differentiation of primary airway epithelial cells. Our SAGM™ Small Airway Epithelial Cell Growth Medium BulletKit CC-3118 does not contain FBS or any other serum.
Missing: stable lines

How many culture/trans-wells does your B-ALI™ Media Kit kit provide?

1 Ampoule of Normal Human Bronchial Epithelial Cells (NHBE) + 1 B-ALI™ Media Kit will make one 24-well plate for at least 1 month of culture & cells will display beating cilia within 25 days.
Missing: stable lines
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