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How should I purify my DNA for Nucleofection® of neurons?
...suppliers. The presence of endotoxins can increase
cell
mortality and this is especially true of sensitive
cells
such as primary neurons. The presence of endotoxins can increase
cell
mortality and this especially true of primary
cells
such as neurons...
Missing:
stable
lines
How do you calculate a population doubling?
A population doubling is the total number of population doublings of a
cell
type since its initiation in vitro. The number of population doubling is calculated using the following equations:
Cell
count at day of passage /
Cell
count 24 hours...
Missing:
stable
lines
What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?
Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example,
cells
could be cultured in Lonza’s BEGM™ medium just prior to differentiation using...
Missing:
stable
lines
What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?
Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example,
cells
could be cultured in Lonza’s SAGM™ medium just prior to differentiation using...
Missing:
stable
lines
What is the purpose of EDTA in trypsin?
EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the
cells
from sticking to each other. If its not there….you would need a higher...
Missing:
stable
lines
What is the Cocoon® Technology?
The Cocoon® Platform utilizes a single use, disposable cassette to functionally close and automate
cell
manufacturing. Primarily developed to automated all steps in CAR-T
cell
manufacturing, the Cocoon® Platform is being used for numerous...
Missing:
stable
lines
Can I use the Nucleofector® Technology for RNAi applications? How do I start?
Yes. The Nucleofector® Technology can be easily applied for any RNAi substrate (siRNA, shRNA, miRNA). You can use the same conditions described in the
cell
type specific Optimized Protocol for DNA vectors (cDNA, shRNA or miRNA expressing plasmids...
Missing:
stable
lines
Can customers buy PBMCs from COVID-19 infected donors?
At this time, we cannot offer any
cells
from active COVID-19 infected donors. We are not completely ruling this out as a possibility for future custom offerings, but at this time we cannot say if/when we may be able to offer this. What we can offer...
Missing:
stable
lines
How is the B-ALI™ Media System performance tested?
The media system is performance tested with our pre-screened NHBE
cells
and that system is guaranteed to form ciliated, mucous-producing
cells
after 3 weeks of culture.
Missing:
stable
lines
What is the amount of DNA needed per well when using the 96-well Shuttle® Device?
The amount depends on the
cell
type and on the DNA construct. As a rule of thumb 400 ng of DNA per well should be applied. However, for some
cells
or constructs even 100 ng may be sufficient.
Missing:
stable
lines
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