Search Results

Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications

Microelectrode array (MEA) technology enables advanced drug screening and “disease-in-a-dish” modeling by measuring the electrical activity of cultured networks of neural or cardiac cells. Recent developments in human stem cell technologies,...

Authors: Clements IP, Millard DC, Nicolini AM, Preyer AJ, Grier R, Heckerling A, Blum RA, Tyler P, McSweeney...

SST Peer Reviewed

Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells

CRISPR/Cas9 has demonstrated a high-efficiency in site-specific gene targeting. However, potential off-target effects of the Cas9 nuclease represent a major safety concern for any therapeutic application. Here, we knock out the Tafazzin gene by...

Authors: Luhan Yang, Dennis Grishin, Gang Wang, John Aach, Cheng-Zhong Zhang, Raj Chari, Jason Homsy, Xuyu...

SST Peer Reviewed

Precursor and mature NGF live tracking: one versus many at a time in the axons.

The classical view of nerve growth factor (NGF) action in the nervous system is linked to its retrograde axonal transport. However, almost nothing is known on the trafficking properties of its unprocessed precursor proNGF, characterized by different...

Authors: De Nadai T, Marchetti L, Di Rienzo C, Calvello M, Signore G3, Di Matteo P, Gobbo F, Turturro S,...

SST Peer Reviewed

Prediction of drug-induced nephrotoxicity and injury mechanisms with human induced pluripotent stem cell-derived cells and machine learning methods.

The renal proximal tubule is a main target for drug-induced toxicity. The prediction of proximal tubular toxicity during drug development remains difficult. Any in vitro methods based on induced pluripotent stem cell-derived renal cells had not been...

Authors: Kandasamy K1, Chuah JK1, Su R2, Huang P1, Eng KG1, Xiong S1, Li Y1, Chia CS1, Loo LH3, Zink D1

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Sequence-specific nucleases such as TALEN and the CRISPR/Cas9 system have so far been used to disrupt, correct or insert transgenes at precise locations in mammalian genomes. We demonstrate efficient 'knock-in' targeted replacement of multi-kilobase...

Authors: Susan M. Byrne, Luis Ortiz, Prashant Mali, John Aach, George M. Church

SST Peer Reviewed

Plumericin inhibits proliferation of vascular smooth muscle cells by blocking STAT3 signaling via S-glutathionylation

The etiology of atherosclerosis and restenosis involves aberrant inflammation and proliferation, rendering compounds with both anti-inflammatory and anti-mitogenic properties as promising candidates for combatting vascular diseases. A recent study...

Authors: Heiss EH, Liu R, Waltenberger B, Khan S, Schachner D, Kollmann P, Zimmermann K, Cabaravdic M, Uhrin...

SST Peer Reviewed

FoxA and LIPG endothelial lipase control the uptake of extracellular lipids for breast cancer growth

The mechanisms that allow breast cancer (BCa) cells to metabolically sustain rapid growth are poorly understood. Here we report that BCa cells are dependent on a mechanism to supply precursors for intracellular lipid production derived from...

Authors: Slebe F, Rojo F, Vinaixa M, García-Rocha M, Testoni G, Guiu M, Planet E, Samino S, Arenas EJ,...

SST Peer Reviewed

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

CRISPR-Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-target effects of the widely used Streptococcus pyogenes Cas9 (SpCas9) are imperfect, possessing...

Authors: Kleinstiver BP, Pattanayak V, Prew MS, Tsai SQ, Nguyen NT, Zheng Z, Joung JK

SST Peer Reviewed

Subcortical cytoskeleton periodicity throughout the nervous system

Superresolution fluorescence microscopy recently revealed a ~190 nm periodic cytoskeleton lattice consisting of actin, spectrin, and other proteins underneath the membrane of cultured hippocampal neurons. Whether the periodic cytoskeleton lattice is...

Authors: D\\\'Este E, Kamin D, Velte C, Göttfert F, Simons M, Hell SW

SST Peer Reviewed

Hsp70 Isoforms Are Essential for the Formation of Kaposi\'s Sarcoma-Associated Herpesvirus Replication and Transcription Compartments.

Kaposi's sarcoma-associated herpesvirus (KSHV) is an oncogenic herpesvirus associated with various AIDS-related malignancies. Like other herpesviruses, multiple processes required for KSHV lytic replication, including viral transcription, viral DNA...

Authors: Baquero-Pérez B, Whitehouse A.

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Optogenetic stimulation of multiwell MEA plates for neural and cardiac applications

Targeted and genome-wide sequencing reveal single nucleotide variations impacting specificity of Cas9 in human stem cells

Precursor and mature NGF live tracking: one versus many at a time in the axons.

Prediction of drug-induced nephrotoxicity and injury mechanisms with human induced pluripotent stem cell-derived cells and machine learning methods.

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

Plumericin inhibits proliferation of vascular smooth muscle cells by blocking STAT3 signaling via S-glutathionylation

FoxA and LIPG endothelial lipase control the uptake of extracellular lipids for breast cancer growth

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects

Subcortical cytoskeleton periodicity throughout the nervous system

Hsp70 Isoforms Are Essential for the Formation of Kaposi\'s Sarcoma-Associated Herpesvirus Replication and Transcription Compartments.