Data Type

Category

+ Show All

Research Area

+ Show All
313 results sorted by

Can the ViaLight™ Plus Kits be used for both proliferation and cytotoxicity assay

Yes.  Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture.  In response to a proliferative signal (e.g. a growth factor) the number of cells...
Missing: stable linesGuideline

Do I need a pure neuronal culture for Nucleofection® ?

In principle, it should be absolutely fine to nucleofect pure neuron cultures (e.g. after purification by Ficoll). Glial cells are not necessary for transfection. What one would have to consider for example, is to get the required number of neurons...
Missing: stable linesGuideline

Can I apply more than one program (transfection condition) per plate with the 96-well Shuttle® System?

With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can...
Missing: stable linesGuideline

How many product samples can I test with one PyroCell® MAT System Kit?

The PyroCell™ MAT System kits contains 3 vials of MAT cells (pMAT cells) and 3 vials of a dedicated MAT Culture Medium Supplement, as well as PeliKine compact ELISA assay components sufficient to run 3x 96-well plates.One vial of pMAT...
Missing: stable linesGuideline

How are Lonza's CD14+ monocytes isolated?

Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using either positive or negative...
Missing: stable linesGuideline

How should I purify my DNA for Nucleofection® of neurons?

...suppliers. The presence of endotoxins can increase cell mortality and this is especially true of sensitive cells such as primary neurons. The presence of endotoxins can increase cell mortality and this especially true of primary cells such as neurons...
Missing: stable linesGuideline

How do you calculate a population doubling?

A population doubling is the total number of population doublings of a cell type since its initiation in vitro. The number of population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours...
Missing: stable linesGuideline

What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?

Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s BEGM™ medium just prior to differentiation using...
Missing: stable linesGuideline

What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using...
Missing: stable linesGuideline

What is the purpose of EDTA in trypsin? 

EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the cells from sticking to each other.  If its not there….you would need a higher...
Missing: stable linesGuideline
PAGE 25

Privacy | Legal | About Lonza

© Lonza. All rights reserved.