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What is the difference between Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium?

Lonza’s SAGM™ medium and the Growth Media portion of Lonza’s S-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s SAGM™ medium just prior to differentiation using...
Missing: stable linesGuideline

Can I use the Nucleofector® Technology for RNAi applications? How do I start?

Yes. The Nucleofector® Technology can be easily applied for any RNAi substrate (siRNA, shRNA, miRNA). You can use the same conditions described in the cell type specific Optimized Protocol for DNA vectors (cDNA, shRNA or miRNA expressing plasmids...
Missing: stable linesGuideline

What is the Cocoon® Technology? 

The Cocoon® Platform utilizes a single use, disposable cassette to functionally close and automate cell manufacturing. Primarily developed to automated all steps in CAR-T cell manufacturing, the Cocoon® Platform is being used for numerous...
Missing: stable linesGuideline

How are Lonza's CD14+ monocytes isolated?

Lonza's CD14+ monocytes are isolated first by obtaining mononuclear cells from peripheral blood via density gradient centrifugation. The CD14+ cells are then isolated form the mononuclear cell population using either positive or negative...
Missing: stable linesGuideline

How do you calculate a population doubling?

A population doubling is the total number of population doublings of a cell type since its initiation in vitro. The number of population doubling is calculated using the following equations:Cell count at day of passage /Cell count 24 hours...
Missing: stable linesGuideline

What is the purpose of EDTA in trypsin? 

EDTA is a chelator of metal ions like calcium and magensium. It is used with trypsin because it helps bind up the divalent cations in the medium. It helps to keep the cells from sticking to each other.  If its not there….you would need a higher...
Missing: stable linesGuideline

Why do plasmids which contain IRES sequences often have lower transfection efficiency?

...Since the downstream gene is usually GFP, the expression level of the GFP reporter will be lower than would be normally seen when compared to plasmids without an IRES-sequence. Thus the percentage of cells expressing detectable GFP fluorescence...
Missing: stable linesGuideline

Can customers buy PBMCs from COVID-19 infected donors? 

At this time, we cannot offer any cells from active COVID-19 infected donors. We are not completely ruling this out as a possibility for future custom offerings, but at this time we cannot say if/when we may be able to offer this. What we can offer...
Missing: stable linesGuideline

What are mycoplasmas?

...and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale, M.arginii, M.fermentans, M.salivarum, M.hyorhinis and A.laidlawii).Mycoplasmas are widespread...
Missing: stable linesGuideline

What is the amount of DNA I can use in my Nucleofection® Experiment?

...of the total reaction volume to avoid significant dilution of the Nucleofector® Solution by substrate buffer. Alternatively, the substrate might be diluted by Nucleofector® Solution.Furthermore, some cells might be sensitive to larger amounts of DNA...
Missing: stable linesGuideline
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