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Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?

The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...
Missing: stable lines

Does Lonza offer Cryopreserved Leukopaks? 

Lonza CellBio Services is pleased to announce the global availability of cryopreserved leukopaks for research use only applications. The cryopreserved leukopaks are manufactured from peripheral blood of normal human donors at a facility...
Missing: stable lines

Does DMSO (ex. brought in with the substrate) affect Nucleofection®?

Most research paper agrees that the upper limit of cell tolerance to DMSO presence in their cell culture media is about 1% final DMSO (this might vary with the cells and media).In the case of the Nucleofection®, the DMSO will likely be transfected...
Missing: stable lines

What is the function of retinoic acid in the BEGM® media? 

In vivo, bronchial epithelial cells differentiate along an abnormal squamous pathway under conditions of retinoid deficiency. The squamous phenotype is characterized by the induction of specific markers such as keratin 13, and the enzymes...
Missing: stable lines

How can I improve Human Hepatocyte viability post Nucleofection™ on the 96-well Shuttle™ device?

In addition to proper cell handling and using culture media optimized for long term culturing of hepatocytes (Lonza Basal medium supplemented with BulletKit), we recommend separating the healthy hepatocytes from the dead cells and debris...
Missing: stable lines

Do you have any recommended methods for detaching human monocytes from the plate for assaying or before Nucleofection®?

We have had good results by incubating the cells in ice cold PBS for 10 minutes and then rinsing the plates. Alternatively, the cells can be detached without a medium change by gently pipetting the cell suspension up and down. Our experience has...
Missing: stable lines

Can the ViaLight™ Plus Kits be used for both proliferation and cytotoxicity assay

Yes.  Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture.  In response to a proliferative signal (e.g. a growth factor) the number of cells...
Missing: stable lines

Do I need a pure neuronal culture for Nucleofection® ?

In principle, it should be absolutely fine to nucleofect pure neuron cultures (e.g. after purification by Ficoll). Glial cells are not necessary for transfection. What one would have to consider for example, is to get the required number of neurons...
Missing: stable lines

How many product samples can I test with one PyroCell® MAT System Kit?

The PyroCell™ MAT System kits contains 3 vials of MAT cells (pMAT cells) and 3 vials of a dedicated MAT Culture Medium Supplement, as well as PeliKine compact ELISA assay components sufficient to run 3x 96-well plates.One vial of pMAT...
Missing: stable lines

How should I purify my DNA for Nucleofection® of neurons?

...suppliers. The presence of endotoxins can increase cell mortality and this is especially true of sensitive cells such as primary neurons. The presence of endotoxins can increase cell mortality and this especially true of primary cells such as neurons...
Missing: stable lines
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