Data Type

Category

+ Show All

Research Area

+ Show All
304 results sorted by

How can I improve Human Hepatocyte viability post Nucleofection™ on the 96-well Shuttle™ device?

In addition to proper cell handling and using culture media optimized for long term culturing of hepatocytes (Lonza Basal medium supplemented with BulletKit), we recommend separating the healthy hepatocytes from the dead cells and debris...
Missing: stable linesGuideline

Can the ViaLight™ Plus Kits be used for both proliferation and cytotoxicity assay

Yes.  Since the ATP levels are precisely maintained in healthy cells, the level of ATP accurately reflects the number of viable cells in a culture.  In response to a proliferative signal (e.g. a growth factor) the number of cells...
Missing: stable linesGuideline

Do you have any recommended methods for detaching human monocytes from the plate for assaying or before Nucleofection®?

We have had good results by incubating the cells in ice cold PBS for 10 minutes and then rinsing the plates. Alternatively, the cells can be detached without a medium change by gently pipetting the cell suspension up and down. Our experience has...
Missing: stable linesGuideline

What is the basic principle of the Nucleofector® Technology?

The Nucleofector® Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell...
Missing: stable linesGuideline

Why is the Nucleofector® Technology ideal for transient protein production ?

For many cell lines in suspension, especially sCHO, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
Missing: stable linesGuideline

Do I need a pure neuronal culture for Nucleofection® ?

In principle, it should be absolutely fine to nucleofect pure neuron cultures (e.g. after purification by Ficoll). Glial cells are not necessary for transfection. What one would have to consider for example, is to get the required number of neurons...
Missing: stable linesGuideline

Can I apply more than one program (transfection condition) per plate with the 96-well Shuttle® System?

With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can...
Missing: stable linesGuideline

How should I purify my DNA for Nucleofection® of neurons?

...suppliers. The presence of endotoxins can increase cell mortality and this is especially true of sensitive cells such as primary neurons. The presence of endotoxins can increase cell mortality and this especially true of primary cells such as neurons...
Missing: stable linesGuideline

What are the difference between the KGM®, KGM®-2, KGM-Gold® BulletKit®?

...to be up to 20% faster and allows for additional population doublings when compared to cells cultured in the KGM™ Medium.In the second optimization of this medium, Lonza further modified the basal media and slightly modified the concentration of several...
Missing: stable linesGuideline

What is the difference between Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium?

Lonza’s BEGM™ medium and the Growth Media portion of Lonza’s B-ALI BulletKit™ Medium are essentially the same and can be used interchangeably (for example, cells could be cultured in Lonza’s BEGM™ medium just prior to differentiation using...
Missing: stable linesGuideline
PAGE 24

Privacy | Legal | About Lonza

© Lonza. All rights reserved.