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What is the difference between each of the types of HUVECs that Lonza offers?
When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the
cells
in?Do you require pooled or single donor
cells
?Do you require additional...
Missing:
stable
lines
How do you recommend that we isolate splenic lymphocytes from mouse spleens for Nucleofection®? Is erythrocyte lysis required?
For the preparation of mouse spleen
cells
we recommend cutting the spleen once and passing the tissue through a 100µM
cell
strainer or steel mesh using a plunger. The
cells
are flushed into a petri dish containing PBS. In order to remove fat,
cell
...
Missing:
stable
lines
What is the difference between the EGM™ and EGM™-MV Growth Media? What is the difference between the EGM™-2 and EGM™-2MV Growth Media?
Lonza endothelial
cell
media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial
cells
(with some exceptions).Lonza endothelial
cell
media not ending with –MV (such...
Missing:
stable
lines
Does Lonza offer Cryopreserved Leukopaks?
Lonza
Cell
Bio Services is pleased to announce the global availability of cryopreserved leukopaks for research use only applications. The cryopreserved leukopaks are manufactured from peripheral blood of normal human donors at a facility...
Missing:
stable
lines
Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?
The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of
cell
free supernatant only!
Cells
MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...
Missing:
stable
lines
How long can Lonzas rat cardiac myocytes survive in culture?
Lonza has observed these
cells
up to 59 days in culture and even at that time, the
cells
displayed beating in syncytium. It is, however, possible that unseen physiological changes may occur to the
cells
during this time. As these
cells
...
Missing:
stable
lines
What is the function of retinoic acid in the BEGM® media?
In vivo, bronchial epithelial
cells
differentiate along an abnormal squamous pathway under conditions of retinoid deficiency. The squamous phenotype is characterized by the induction of specific markers such as keratin 13, and the enzymes...
Missing:
stable
lines
Does DMSO (ex. brought in with the substrate) affect Nucleofection®?
Most research paper agrees that the upper limit of
cell
tolerance to DMSO presence in their
cell
culture media is about 1% final DMSO (this might vary with the
cells
and media).In the case of the Nucleofection®, the DMSO will likely be transfected...
Missing:
stable
lines
How can I improve Human Hepatocyte viability post Nucleofection™ on the 96-well Shuttle™ device?
In addition to proper
cell
handling and using culture media optimized for long term culturing of hepatocytes (Lonza Basal medium supplemented with BulletKit), we recommend separating the healthy hepatocytes from the dead
cells
and debris...
Missing:
stable
lines
Can the ViaLight™ Plus Kits be used for both proliferation and cytotoxicity assay
Yes. Since the ATP levels are precisely maintained in healthy
cells
, the level of ATP accurately reflects the number of viable
cells
in a culture. In response to a proliferative signal (e.g. a growth factor) the number of
cells
...
Missing:
stable
lines
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