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Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?
SARS-Cov-2 enters target
cells
through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target
cells
. ACE2 is an important regulator of the immune response and inflammation. It is expressed in multiple...
Missing:
stable
lines
What is the basic principle of the Nucleofector® Technology?
The Nucleofector® Technology is a novel transfection method especially designed for the needs of primary
cells
and difficult-to-transfect
cell
lines
. It is a non-viral method based on a unique combination of electrical parameters and
cell
...
Missing:
stable
Do we sell the Nucleofector® Solution (or any other component of the Nucleofector® Kit) separately?
We don't sell the individual components of the kits separately because our product
line
is a kit concept
line
to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.
Missing:
stable
cell
Can I use the Nucleofector® 2b Solutions and Optimized Protocols with the 4D-Nucleofector® X-Unit or the 96-well Shuttle® System?
No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary
cells
. For
cell
lines
, three...
Missing:
stable
What is the proof that DNA enters the nucleus during Nucleofection®?
...is a prerequisite for DNA entry into the nucleus. Until now, the only method to transfect non-dividing
cells
was by using a viral vector system. 2.) In nucleofected
cells
gene expression can be detected shortly after Nucleofection®. In
cell
lines
, the transfected...
Missing:
stable
How do you ship the Pyro
Cell
® Monocyte Activation Test (MAT) Kits?
Depending on the scheduled transfer time, cryopreserved goods are either transported in a styrofoam box with dry ice, or in the vapor phase of liquid nitrogen (LN). Upon receiving, ensure immediate storage of components at = -80°C.If dry...
Missing:
stable
lines
What are the difference between the KGM®, KGM®-2, KGM-Gold® BulletKit®?
...is trying to reproduce a scholarly article where KGM® Medium or KGM®-2 Medium was used with either a
cell
line
,
cells
they isolated on their own, or
cells
from another source.On the below datasheet, you can see the supplements included in each...
Missing:
stable
Where do I find information on the CytoSMART™ System?
Lonza has discontinued to sell the CytoSMART™ System. CytoSMART Technologies has taken over sole support of CytoSMART™ 2 and CytoSMART™ Lux 10X Units.For technical and other questions, please contact CytoSMART Technologies’ support board. More...
Missing:
stable
lines
What is the difference between each of the types of HUVECs that Lonza offers?
When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the
cells
in?Do you require pooled or single donor
cells
?Do you require additional...
Missing:
stable
lines
How do you recommend that we isolate splenic lymphocytes from mouse spleens for Nucleofection®? Is erythrocyte lysis required?
For the preparation of mouse spleen
cells
we recommend cutting the spleen once and passing the tissue through a 100µM
cell
strainer or steel mesh using a plunger. The
cells
are flushed into a petri dish containing PBS. In order to remove fat,
cell
...
Missing:
stable
lines
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