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What optimization is necessary to get the Nucleofector® Technology to work?

None. For each cell type in our product list we offer a cell-type specific solution and thoroughly optimized electrical parameters. These are already pre-programmed in the Nucleofector® Device. Additionally our optimized protocols give you...
Missing: stable linesGuideline

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

...Lonza will only isolate one cell type from a single piece of tissue and all product from that tissue will be processed at the same time. In such a circumstance, there would only be one TAN (for the single, initial piece of tissue) and one lot/batch...
Missing: stable linesGuideline

I see activation of my monocytes (or macrophages or DCs) following Nucleofection®. Why is this? What can I do to address this problem?

We have examined the effects of Nucleofection® (without DNA) on these cells and have not observed significant activation. This indicates that neither the Nucleofector® Solution, the Nucleofector® Program nor the recovery medium...
Missing: stable linesGuideline

Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?

SARS-Cov-2 enters target cells through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target cells1 . ACE2 is an important regulator of the immune response and inflammation. It is expressed...
Missing: stable linesGuideline

How do you ship the PyroCell® Monocyte Activation Test (MAT) Kits?

Depending on the scheduled transfer time, cryopreserved goods are either transported in a styrofoam box with dry ice, or in the vapor phase of liquid nitrogen (LN). Upon receiving, ensure immediate storage of components at = -80°C.If dry...
Missing: stable linesGuideline

Where do I find information on the CytoSMART™ System?

Lonza has discontinued to sell the CytoSMART™ System. CytoSMART Technologies has taken over sole support of CytoSMART™ 2 and CytoSMART™ Lux 10X Units.For technical and other questions, please contact CytoSMART Technologies’ support board. More...
Missing: stable linesGuideline

How do you recommend that we isolate splenic lymphocytes from mouse spleens for Nucleofection®? Is erythrocyte lysis required?

For the preparation of mouse spleen cells we recommend cutting the spleen once and passing the tissue through a 100µM cell strainer or steel mesh using a plunger. The cells are flushed into a petri dish containing PBS. In order to remove fat, cell...
Missing: stable linesGuideline

What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...
Missing: stable linesGuideline

Why do plasmids that contain LTR sequences often have lower transfection efficiency?

...are transfected into cells by Nucleofection®, the expression of the cloned genes is suppressed by the cell. Although the mechanism of suppression is not known, we have certain ideas on what might be happening. Since with Nucleofection®, the DNA is introduced...
Missing: stable linesGuideline

What is the difference between the EGM™ and EGM™-MV Growth Media? What is the difference between the EGM™-2 and EGM™-2MV Growth Media?

Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions).Lonza endothelial cell media not ending with –MV (such...
Missing: stable linesGuideline
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