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What is the difference between each of the types of HUVECs that Lonza offers?

When choosing between the various types of HUVECs that Lonza offers, the choice can be made by answering the following questions:What media do you intend to culture the cells in?Do you require pooled or single donor cells?Do you require additional...
Missing: stable linesGuideline

Why do plasmids that contain LTR sequences often have lower transfection efficiency?

...are transfected into cells by Nucleofection®, the expression of the cloned genes is suppressed by the cell. Although the mechanism of suppression is not known, we have certain ideas on what might be happening. Since with Nucleofection®, the DNA is introduced...
Missing: stable linesGuideline

How do you recommend that we isolate splenic lymphocytes from mouse spleens for Nucleofection®? Is erythrocyte lysis required?

For the preparation of mouse spleen cells we recommend cutting the spleen once and passing the tissue through a 100µM cell strainer or steel mesh using a plunger. The cells are flushed into a petri dish containing PBS. In order to remove fat, cell...
Missing: stable linesGuideline

What is the difference between the EGM™ and EGM™-MV Growth Media? What is the difference between the EGM™-2 and EGM™-2MV Growth Media?

Lonza endothelial cell media ending with –MV (such as the EGM™-MV and EGM™-2MV Growth Media) are typically designated for use with microvascular endothelial cells (with some exceptions).Lonza endothelial cell media not ending with –MV (such...
Missing: stable linesGuideline

Does Lonza offer Cryopreserved Leukopaks? 

Lonza CellBio Services is pleased to announce the global availability of cryopreserved leukopaks for research use only applications. The cryopreserved leukopaks are manufactured from peripheral blood of normal human donors at a facility...
Missing: stable linesGuideline

Do you have recommendations for the type of samples used for the MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Detection Kit? And do you have recommendations related to sample storage?

The MycoAlert® Mycoplasma Detection Kit and the MycoAlert® PLUS Mycoplasma Assay require the use of cell free supernatant only! Cells MUST be removed at 200xg for 5 mins.For optimal assay performance, supernatant should be tested...
Missing: stable linesGuideline

What is the function of retinoic acid in the BEGM® media? 

In vivo, bronchial epithelial cells differentiate along an abnormal squamous pathway under conditions of retinoid deficiency. The squamous phenotype is characterized by the induction of specific markers such as keratin 13, and the enzymes...
Missing: stable linesGuideline

How long can Lonzas rat cardiac myocytes survive in culture?

Lonza has observed these cells up to 59 days in culture and even at that time, the cells displayed beating in syncytium. It is, however, possible that unseen physiological changes may occur to the cells during this time. As these cells...
Missing: stable linesGuideline

What is the proof that DNA enters the nucleus during Nucleofection®?

There are two facts that prove that the DNA is directly transported into the nucleus: 1.) Using the Nucleofector® Technology, transfection of non-dividing cells is possible. With conventional non-viral transfection methods cell division...
Missing: stable linesGuideline

Does DMSO (ex. brought in with the substrate) affect Nucleofection®?

Most research paper agrees that the upper limit of cell tolerance to DMSO presence in their cell culture media is about 1% final DMSO (this might vary with the cells and media).In the case of the Nucleofection®, the DMSO will likely be transfected...
Missing: stable linesGuideline
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