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Why is the Nucleofector® Technology ideal for transient protein production ?

For many cell lines in suspension, especially sCHO, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
Missing: stable

I see activation of my monocytes (or macrophages or DCs) following Nucleofection®. Why is this? What can I do to address this problem?

We have examined the effects of Nucleofection® (without DNA) on these cells and have not observed significant activation. This indicates that neither the Nucleofector® Solution, the Nucleofector® Program nor the recovery medium...
Missing: stable lines

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
Missing: stable

Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?

SARS-Cov-2 enters target cells through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target cells1 . ACE2 is an important regulator of the immune response and inflammation. It is expressed...
Missing: stable lines

What is the basic principle of the Nucleofector® Technology?

The Nucleofector® Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell...
Missing: stable

Can I use the Nucleofector® 2b Solutions and Optimized Protocols with the 4D-Nucleofector® X-Unit or the 96-well Shuttle® System?

No. The 4D-Nucleofector and 96-well Shuttle® System uses specifically optimized electrical parameters and solutions. Five different 4D-Nucleofector or 96-well Shuttle® Solutions are available for primary cells. For cell lines, three...
Missing: stable

What is the proof that DNA enters the nucleus during Nucleofection®?

...is a prerequisite for DNA entry into the nucleus. Until now, the only method to transfect non-dividing cells was by using a viral vector system. 2.) In nucleofected cells gene expression can be detected shortly after Nucleofection®. In cell lines, the transfected...
Missing: stable

Do we sell the Nucleofector® Solution (or any other component of the Nucleofector® Kit) separately?

We don't sell the individual components of the kits separately because our product line is a kit concept line to ensure the use of certified products with quality controlled lot numbers that guarantee optimal results.
Missing: stable cell

What are the difference between the KGM®, KGM®-2, KGM-Gold® BulletKit®?

...is trying to reproduce a scholarly article where KGM® Medium or KGM®-2 Medium was used with either a cell line, cells they isolated on their own, or cells from another source.On the below datasheet, you can see the supplements included in each...
Missing: stable

Where do I find information on the CytoSMART™ System?

Lonza has discontinued to sell the CytoSMART™ System. CytoSMART Technologies has taken over sole support of CytoSMART™ 2 and CytoSMART™ Lux 10X Units.For technical and other questions, please contact CytoSMART Technologies’ support board. More...
Missing: stable lines
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