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How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen...
Missing: cell lines

How should I handle the reconstituted Lonza LAL PYROGENT™ Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...
Missing: cell lines

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

...Lonza will only isolate one cell type from a single piece of tissue and all product from that tissue will be processed at the same time. In such a circumstance, there would only be one TAN (for the single, initial piece of tissue) and one lot/batch...
Missing: stable lines

Can I apply more than one program (transfection condition) per plate with the 96-well Shuttle® System?

With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can...
Missing: stable

I see activation of my monocytes (or macrophages or DCs) following Nucleofection®. Why is this? What can I do to address this problem?

We have examined the effects of Nucleofection® (without DNA) on these cells and have not observed significant activation. This indicates that neither the Nucleofector® Solution, the Nucleofector® Program nor the recovery medium...
Missing: stable lines

Why do plasmids that contain LTR sequences often have lower transfection efficiency?

...This is particularly true in primary cells, which have all of the normal cellular self-defense mechanisms still intact, and is true to a lesser extent with cell lines. During the process of immortalization or transformation, many cell lines lose parts their DNA...
Missing: stable

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
Missing: stable

Why is the Nucleofector® Technology ideal for transient protein production ?

For many cell lines in suspension, especially sCHO, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
Missing: stable

Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?

SARS-Cov-2 enters target cells through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target cells1 . ACE2 is an important regulator of the immune response and inflammation. It is expressed...
Missing: stable lines

What is the basic principle of the Nucleofector® Technology?

The Nucleofector® Technology is a novel transfection method especially designed for the needs of primary cells and difficult-to-transfect cell lines. It is a non-viral method based on a unique combination of electrical parameters and cell...
Missing: stable
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