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What is the difference between the FBM™ Fibroblast Growth Basal Medium CC-3131 and the SCBM™ Stromal Cell Growth Basal Medium CC-3204? Can the FBM™ Basal Medium and the SCBM™ Basal Medium be used interchangeable? Is there a phenol red free version of the FBM™ Basal Medium?

The FBM™ Basal Medium and the SCBM™ Basal Medium are essentially the same except that the FBM™ Basal Medium contains phenol red and the SCBM™ Basal Medium does not. The FBM™ Basal Medium and the SCBM™ Basal Medium can always be used interchangeably...
Missing: stable lines

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

...components are very stable, but you should discard any reconstituted reagents. We recommend performing an initial qualification assay to confirm that the reagents still meet the performance characteristics required by the FDA. The PyroGene rFC assay kit must...
Missing: cell lines

In my Nucleofection® Experiment, how critical is the time point for analysis post transfection?

...protein (like luciferase) analysis should be done at 6-18 hours after Nucleofection®. For a more stable protein, such as GFP, one can wait to start analysis for up to 24 hours or longer post Nucleofection®. As Nucleofection® delivers DNA directly...
Missing: cell lines

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen...
Missing: cell lines

How should I handle the reconstituted Lonza LAL PYROGENT™ Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...
Missing: cell lines

Can I apply more than one program (transfection condition) per plate with the 96-well Shuttle® System?

With the 96-well Shuttle® system, it is possible to apply up to 96 different programs (transfection conditions) per 96-well Nucleocuvette® Plate. Therefore, optimization of Nucleofection® Conditions for a particular cell line can...
Missing: stable

Why do plasmids that contain LTR sequences often have lower transfection efficiency?

...This is particularly true in primary cells, which have all of the normal cellular self-defense mechanisms still intact, and is true to a lesser extent with cell lines. During the process of immortalization or transformation, many cell lines lose parts their DNA...
Missing: stable

Why is the Nucleofector® Technology ideal for transient protein production ?

For many cell lines in suspension, especially sCHO, conventional transfection methods are very limited in efficiency. For protein production, suspension cells are favored over adherent cells because they are much easier to cultivate. Unlike...
Missing: stable

Can I use the settings recommended for the Nucleofector® I/II or 2b System with my 4D-Nucleofector® System?

No, the 4D-Nucleofector® is optimized for conductive polymer electrodes, while the Nucleofector® I/II or 2b System is working with electrodes made of aluminium.We recommend doing a quick cell line optimization using our Cell Line...
Missing: stable

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

...product. One TAN can be associated with one or multiple lot/batch numbers, depending on how the tissue is processed.Common scenarios include:Single tissue, single product:If one cell type is isolated from one tissue sample and processed in a single...
Missing: stable lines
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