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Can you use the AdipoRed™ Assay on cells other than preadipocytes?

Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.

How many 100 µl Nucleocuvettes® can be transfected in parallel using the 4D-Nucleofector® System?

The 4D-Nucleofector® X Unit can work with two 100µl Nucleocuvettes® in parallel. However, the operation software allows defining up to 50 Nucleocuvettes® per experiment. These cuvettes will be transfected by the system continuously in...

Does the recommended media for each of the diabetic cells contain glucose?

The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations.

Can I optimize the transfection efficiency or cell viability after the 4D-Nucleofector® Cell Line Optimization any further?

We offer a program fine tuning matrix for Optimization of Nucleofection® Conditions - A Short Guideline (lonza.com)Based on your current most satisfactory pulse(s) it provides guidelines which further programs to test to either increase cell...

Is the viability of cells from diseased or diabetic donors less than cells from normal donors?

We guarantee > 70 % viability for cells from both diseased/diabetic donors and normal donors.

Do we have any Diseased Human Bronchial/Tracheal Epithelial Cells (DHBE) screened for Air Liquid Interface (ALI) differentiation using the B-ALI™ BulletKit™?

Yes. All our DHBEs are also offered in a version screened for ALI differentiation. The lots which do pass this additional ALI testing are electronically designated by adding an “S” to the end of the original catalog number.

How many passages can I use the Clonetics™ diseased cells?

Proliferative capacity of Lonza's Clonetics™ diseased cells is tested through one or more passages (depending on the cell type) for information only.It is NOT a specification where a minimum number of doublings must be obtained in order to pass...

What is a Clonetics™ or Poietics™ Basal Medium?

The Clonetics™ and Poietics™ Basal Media are primarily composed of trace metals, amino acids, vitamins, salts, and water. The basal medium contains no growth factors or antibiotics and is not sufficient to support cell attachment and growth. To the...

Do we know the severity of the asthma or the genotype of the cystic fibrosis donors?

Generally, information on the severity of asthma is not available. For our diseased cells from donors with cystic fibrosis, we usually have information on the genotype of the donor. Please contact our scientific support department for further...

What is the purpose of the 5-bromo-2'-deoxyuridine (BrdU) when culturing rat cardiac myocytes?

5-bromo-2'-deoxyuridine (BrdU) is very efficient at inhibiting the proliferation of fibroblasts which are natively present at the time of isolation. Without the addition of the BrdU, the fibroblasts would overtake the culture.

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