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What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.

What cells make up the population of cells in Lonza's bone marrow stromal cells?

The stromal layer is composed of many cell types including fibroblasts, mesenchymal stem cells, adipocytes, endothelial cells, and macrophages.

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...

Where are Lonza’s NHOst CC-2538 cells isolated from?

Lonza obtains our Normal Human Osteoblasts (NHOst) from the spongy section of the bone head of ribs, femur, radius, fibula or tibia.If you would like to know, from which specific bone your lot of NHOst has been isolated, just contact our Scientific...

What nitrocellulose do you recommend for coating plates when culturing Lonza's rat cardiac myocytes?

Internally we use catalog number 162-0113 from Bio-Rad.

Where are Lonza’s NHAC-kn cells isolated from?

Lonza obtains our Normal Human Articular Chondrocytes (NHAC-kn) from the weight bearing cartilage of the knee (hyaline cartilage)

Do you have a transfection protocol for cells from clouded leopard or fishing cat?

Yes, we have a excellent reference for this question. The clouded leopard (Neofelis nebulosa) is currently in vulnerable status according to the International Union for Conservation of Nature red list (2017). The Zoological Park Organziation of...

What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501

Which program should I use for co-transfection of RNP and CRISPR/Cas9 complexes ? The paper "Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells from Aikiko Seki and Sascha Rutz showed different programs as you have in the Lonza optimized Nucleofector Protocols?

Their publication in the Journal of Experimental Medicine is an advanced optimization for gene knock out in primary human and mouse T cells. The final protocol achieved 85-98% gene knock out for several tested receptors.The group has tested and...

Where are Lonza’s HSMM cells isolated from?

Lonza obtains our Human Skeletal Muscle Myoblasts( HSMM), both diseased and normal, from quadriceps or psoas muscle tissue.

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