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I would like to use the dipping electrode array for less than 24 wells. How can I keep non-required dipping electrodes clean?

We would recommend to prepare the culture plate as such that cells are only plated into those wells that should be transfected and leave further wells empty.After withdrawing the dipping electrode array from the plate you can wipe off residual liquid...

How many 100 µl Nucleocuvettes® can be transfected in parallel using the 4D-Nucleofector® System?

The 4D-Nucleofector® X Unit can work with two 100µl Nucleocuvettes® in parallel. However, the operation software allows defining up to 50 Nucleocuvettes® per experiment. These cuvettes will be transfected by the system continuously in...

Can I optimize the transfection efficiency or cell viability after the 4D-Nucleofector® Cell Line Optimization any further?

We offer a program fine tuning matrix for Optimization of Nucleofection® Conditions - A Short Guideline (lonza.com)Based on your current most satisfactory pulse(s) it provides guidelines which further programs to test to either increase cell...

The basic protocol for adherent Nucleofection« of neurons using the 4D-Nucleofector® Y Unit recommends to plate 150,000 freshly isolated or 300,000 cryopreserved neurons. What can I do if do not have enough cells to perform several samples?

Once you have found your optimal conditions you can always try to reduce the recommended cell number. To find out if lower cell numbers have been successfully used already, please check our cell data base. If you would like to reduce the cell number...

Where are Lonza’s Human Skeletal Muscle Myoblasts (HSMM) cells isolated from?

The Human Skeletal Muscle Myoblasts are sourced from normal post-gestational tissue of cadavers from 1 day to 40 years old. The HSMMs are isolated from the quadriceps or psoas muscle.

How many mycoplasma species exist? Which are the usual suspects in cell culture?

More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale,...

I transfected neurons using the 4D-Nucleofector® Y-Unit. After Nucleofection®, there were precipitates in my culture. What can I do?

Most likely there was too much Nucleofector® Solution left before medium addition. Please try to aspirate solution after Nucleofection® as complete as possible without disturbing cell adherence, but add medium quickly to avoid cells getting...

How can Lonza’s Human Skeletal Muscle Myoblasts (HSMM) be differentiated to produce desmin?

Human Skeletal Muscle Myoblasts usually begin to form multinucleated myotubes when the culture reaches 50-70% confluence. Myotubes are formed by the fusion of several to many myoblasts. Addition of differentiation medium, when the culture reaches...

Approximately what size are the Coronary Artery Smooth Muscle Cells (CASMCs)?

Lonza’s R&D estimates that the CASMC’s are around 30 µm in diameter when rounded/in suspension.

Where are Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) isolated from?

While Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) are isolated from either the ascending or descending aorta, it is usually the ascending aorta that is used. If the heart comes with the aorta, which happens most of the time, then the AoSMC are...

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