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What is the difference between the FBM™ Fibroblast Growth Basal Medium CC-3131 and the SCBM™ Stromal Cell Growth Basal Medium CC-3204? Can the FBM™ Basal Medium and the SCBM™ Basal Medium be used interchangeable? Is there a phenol red free version of the FBM™ Basal Medium?

The FBM™ Basal Medium and the SCBM™ Basal Medium are essentially the same except that the FBM™ Basal Medium contains phenol red and the SCBM™ Basal Medium does not. The FBM™ Basal Medium and the SCBM™ Basal Medium can always be used interchangeably...
Missing: stable linesGuideline

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

...components are very stable, but you should discard any reconstituted reagents. We recommend performing an initial qualification assay to confirm that the reagents still meet the performance characteristics required by the FDA. The PyroGene rFC assay kit must...
Missing: cell linesGuideline

In my Nucleofection® Experiment, how critical is the time point for analysis post transfection?

...protein (like luciferase) analysis should be done at 6-18 hours after Nucleofection®. For a more stable protein, such as GFP, one can wait to start analysis for up to 24 hours or longer post Nucleofection®. As Nucleofection® delivers DNA directly...
Missing: cell linesGuideline

How do I handle my Lonza Control Standard Endotoxin (CSE) for Bacterial Endotoxin Testing (BET)?

Lyophilized E. coli endotoxin should be stored at 2-8°C. Endotoxin solutions are generally very stable upon storage except at more dilute concentrations. Rehydrated E. coli endotoxin should be stored refrigerated only (at 2-8°C and not frozen...
Missing: cell linesGuideline

How should I handle the reconstituted Lonza LAL PYROGENT™ Gel Clot Bacterial Endotoxin Testing (BET) Reagent?

Rehydrated LAL reagent may be stored for as long as 24 hours, but it must be kept at 2-8 °C. Do not pre-incubate lysate in the LAL test tubes before adding samples to them. This is not necessary and may cause sensitivity loss in the LAL reagent. If...
Missing: cell linesGuideline

What optimization is necessary to get the Nucleofector® Technology to work?

None. For each cell type in our product list we offer a cell-type specific solution and thoroughly optimized electrical parameters. These are already pre-programmed in the Nucleofector® Device. Additionally our optimized protocols give you...
Missing: stable linesGuideline

What is the difference between a Tissue Acquisition Number (TAN) and a lot/batch number?

...Lonza will only isolate one cell type from a single piece of tissue and all product from that tissue will be processed at the same time. In such a circumstance, there would only be one TAN (for the single, initial piece of tissue) and one lot/batch...
Missing: stable linesGuideline

I see activation of my monocytes (or macrophages or DCs) following Nucleofection®. Why is this? What can I do to address this problem?

We have examined the effects of Nucleofection® (without DNA) on these cells and have not observed significant activation. This indicates that neither the Nucleofector® Solution, the Nucleofector® Program nor the recovery medium...
Missing: stable linesGuideline

Pathogenic human coronaviruses such as severe acute respiratory syndrome CoV (SARS-CoV) predominantly infect lower airways and can cause fatal pneumonia. What kind of products you have to support this research area?

SARS-Cov-2 enters target cells through the (angiotensin converting enzyme) - receptor leading to infections of ACE-2 expressing target cells1 . ACE2 is an important regulator of the immune response and inflammation. It is expressed...
Missing: stable linesGuideline

Where do I find information on the CytoSMART™ System?

Lonza has discontinued to sell the CytoSMART™ System. CytoSMART Technologies has taken over sole support of CytoSMART™ 2 and CytoSMART™ Lux 10X Units.For technical and other questions, please contact CytoSMART Technologies’ support board. More...
Missing: stable linesGuideline
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