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Did you know that by combining ToxiLight™ Non-Destructive Cytotoxicity BioAssay with ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay you can assess proliferation, cytotoxicity and even cytostasis?

Proliferation shows increased Vialight™ Plus RLUs (Relative Light Units) but NO increase in ToxiLight™ RLUs*Cytotoxicity shows decreased Vialight™ Plus RLUs and increased ToxiLight™ RLUs*Cytostasis (where the cells are alive but unable...
Missing: stable lines

When performing the PyroCell® Monocyte Activation Test, can I use a different cell culture medium than the IMDM (Iscove’s modified Dulbecco’s medium) recommended by Lonza?

Yes. However, the recommended medium has been validated with the PeliKine Human IL-6 ELISA Rapid Kit included in the PyroCell® MAT System. Please note that experience with alternative cell culture medium is limited. The IMDM 12-722F from Lonza...
Missing: stable lines

Is it necessary to remove antibiotics for some time from the cell culture medium before taking sample to test medium for presence of mycoplasma using the MycoAlert® Mycoplasma Detection Kit?

The MycoAlert® Mycoplasma Detection Assay was designed to “fit” in with normal tissue culturing practices. It was developed in fully supplemented culture media containing antibiotics and DOES NOT require a period of culture without antibiotics...
Missing: stable lines

Which program should I use for co-transfection of RNP and CRISPR/Cas9 complexes ? The paper "Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T cells from Aikiko Seki and Sascha Rutz showed different programs as you have in the Lonza optimized Nucleofector Protocols?

Their publication in the Journal of Experimental Medicine is an advanced optimization for gene knock out in primary human and mouse T cells. The final protocol achieved 85-98% gene knock out for several tested receptors.The group has tested...
Missing: stable lines

The basic protocol for adherent Nucleofection« of neurons using the 4D-Nucleofector® Y Unit recommends to plate 150,000 freshly isolated or 300,000 cryopreserved neurons. What can I do if do not have enough cells to perform several samples?

Once you have found your optimal conditions you can always try to reduce the recommended cell number. To find out if lower cell numbers have been successfully used already, please check our cell data base. If you would like to reduce the cell number...
Missing: stable lines

DonorPlex™ Pooled Donor Human Hepatocytes: on the certificates of analysis, the percentage of male vs female donors in the pool or caucasian vs asian vs african american donors is indicated. Do the percentages refer to the numbers of donors in the lot or are those the percentage of cells in the pool?

The indicated percentages refer to the numbers of donors in the pool. When pooling, we are targeting multiple variables so the ratio of each donor may be different to achieve a pool that meets the targets specification (for example a specific...
Missing: stable lines

What is the difference between the FBM™ Fibroblast Growth Basal Medium CC-3131 and the SCBM™ Stromal Cell Growth Basal Medium CC-3204? Can the FBM™ Basal Medium and the SCBM™ Basal Medium be used interchangeable? Is there a phenol red free version of the FBM™ Basal Medium?

The FBM™ Basal Medium and the SCBM™ Basal Medium are essentially the same except that the FBM™ Basal Medium contains phenol red and the SCBM™ Basal Medium does not. The FBM™ Basal Medium and the SCBM™ Basal Medium can always be used interchangeably...
Missing: stable lines

When using the PyroCell® MAT System Kit: Can I store remaining culture supernatant from the MAT stimulation?

Yes, for the IL-6 cytokine measurement. Remaining cell culture supernatant may be frozen at -20°C or -80°C for future use, e.g. retesting. Storage time of the frozen supernatant may be also dependent on the test substance used for stimulation...
Missing: lines

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

...components are very stable, but you should discard any reconstituted reagents. We recommend performing an initial qualification assay to confirm that the reagents still meet the performance characteristics required by the FDA. The PyroGene rFC assay kit must...
Missing: cell lines

In my Nucleofection® Experiment, how critical is the time point for analysis post transfection?

...protein (like luciferase) analysis should be done at 6-18 hours after Nucleofection®. For a more stable protein, such as GFP, one can wait to start analysis for up to 24 hours or longer post Nucleofection®. As Nucleofection® delivers DNA directly...
Missing: cell lines
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