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Are the Rat Aortic Smooth Muscle
Cell
s R-ASM-580 derived from single donor or does each lot contain a pool from several animals?
The R-ASM-580 per lot contain pooled
cells
from 4-5 male Sprague Dawley rats
Missing:
stable
lines
I transfected neurons using the 4D-Nucleofector® Y unit. During analysis I observed areas with low or no
cell
density, even in my no pulse control sample.
Cells
might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film...
Missing:
stable
lines
How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need
cell
s or just supernatant?
The standard protocol calls for taking 2 ml of culture media, spinning down any
cells
and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing:
stable
lines
Did you know that by combining ToxiLight™ Non-Destructive Cytotoxicity BioAssay with ViaLight™ Plus
Cell
Proliferation and Cytotoxicity BioAssay you can assess proliferation, cytotoxicity and even cytostasis?
Proliferation shows increased Vialight™ Plus RLUs (Relative Light Units) but NO increase in ToxiLight™ RLUs*Cytotoxicity shows decreased Vialight™ Plus RLUs and increased ToxiLight™ RLUs*Cytostasis (where the
cells
are alive but unable...
Missing:
stable
lines
When performing the Pyro
Cell
® Monocyte Activation Test, can I use a different
cell
culture medium than the IMDM (Iscove’s modified Dulbecco’s medium) recommended by Lonza?
Yes. However, the recommended medium has been validated with the PeliKine Human IL-6 ELISA Rapid Kit included in the Pyro
Cell
® MAT System. Please note that experience with alternative
cell
culture medium is limited. The IMDM 12-722F from Lonza...
Missing:
stable
lines
Is it necessary to remove antibiotics for some time from the
cell
culture medium before taking sample to test medium for presence of mycoplasma using the MycoAlert® Mycoplasma Detection Kit?
The MycoAlert® Mycoplasma Detection Assay was designed to “fit” in with normal tissue culturing practices. It was developed in fully supplemented culture media containing antibiotics and DOES NOT require a period of culture without antibiotics...
Missing:
stable
lines
Which program should I use for co-transfection of RNP and CRISPR/Cas9 complexes ? The paper "Optimized RNP transfection for highly efficient CRISPR/Cas9-mediated gene knockout in primary T
cell
s from Aikiko Seki and Sascha Rutz showed different programs as you have in the Lonza optimized Nucleofector Protocols?
Their publication in the Journal of Experimental Medicine is an advanced optimization for gene knock out in primary human and mouse T
cells
. The final protocol achieved 85-98% gene knock out for several tested receptors.The group has tested...
Missing:
stable
lines
The basic protocol for adherent Nucleofection« of neurons using the 4D-Nucleofector® Y Unit recommends to plate 150,000 freshly isolated or 300,000 cryopreserved neurons. What can I do if do not have enough
cell
s to perform several samples?
Once you have found your optimal conditions you can always try to reduce the recommended
cell
number. To find out if lower
cell
numbers have been successfully used already, please check our
cell
data base. If you would like to reduce the
cell
number...
Missing:
stable
lines
DonorPlex™ Pooled Donor Human Hepatocytes: on the certificates of analysis, the percentage of male vs female donors in the pool or caucasian vs asian vs african american donors is indicated. Do the percentages refer to the numbers of donors in the lot or are those the percentage of
cell
s in the pool?
The indicated percentages refer to the numbers of donors in the pool. When pooling, we are targeting multiple variables so the ratio of each donor may be different to achieve a pool that meets the targets specification (for example a specific...
Missing:
stable
lines
When using the Pyro
Cell
® MAT System Kit: Can I store remaining culture supernatant from the MAT stimulation?
Yes, for the IL-6 cytokine measurement. Remaining
cell
culture supernatant may be frozen at -20°C or -80°C for future use, e.g. retesting. Storage time of the frozen supernatant may be also dependent on the test substance used for stimulation...
Missing:
lines
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