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In your optimized Nucleofection® Protocols for MDA-MB-231 and MDA-MB-468, you say to culture the cells without CO2. Why?

The media recommended for these cells is Leibovitz's L-15 medium which does not contain sodium bicarbonate to act as a buffer for the carbonic acid that would normally form in the presence of CO2. Furthermore, if the cells are cultured in Leibovitz...
Missing: stable lines

Do you know if treating cells with 10mM MgCl2 would interfere with the ToxiLight™ Non-Destructive Cytotoxicity BioAssay?

Magnesium (Mg) is a cofactor for both luciferase and Adenylate Kinase (AK) and will speed up their reactions, but this amount of MgCl2 wouldn't make a significant difference.
Missing: stable lines

Can I use the ViaLight™ Plus Assay also, if my cells are cultured in 24well plates, 6well plates or 12 well plates?

To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the cell lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl cell lysis). Wait 10 minutes for the lysis . Then follow...
Missing: stable lines

DNA-purity and Nucleofection®: Can low A260:A280 ratios lead to both reduced transfection efficiency and cell viability?

Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio. It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
Missing: stable lines

How does Lonza perform the cell count on the fresh, unprocessed bone marrow? Does Lonza guarantee a minimum viability for the fresh, unprocessed bone marrow?

Lonza use a Coulter Counter to perform the total cell count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.
Missing: stable lines

Are the Rat Aortic Smooth Muscle Cells R-ASM-580 derived from single donor or does each lot contain a pool from several animals?

The R-ASM-580 per lot contain pooled cells from 4-5 male Sprague Dawley rats
Missing: stable lines

I transfected neurons using the 4D-Nucleofector® Y unit. During analysis I observed areas with low or no cell density, even in my no pulse control sample.

Cells might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film...
Missing: stable lines

How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need cells or just supernatant?

The standard protocol calls for taking 2 ml of culture media, spinning down any cells and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing: stable lines

Did you know that by combining ToxiLight™ Non-Destructive Cytotoxicity BioAssay with ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay you can assess proliferation, cytotoxicity and even cytostasis?

Proliferation shows increased Vialight™ Plus RLUs (Relative Light Units) but NO increase in ToxiLight™ RLUs*Cytotoxicity shows decreased Vialight™ Plus RLUs and increased ToxiLight™ RLUs*Cytostasis (where the cells are alive but unable...
Missing: stable lines

Is it necessary to remove antibiotics for some time from the cell culture medium before taking sample to test medium for presence of mycoplasma using the MycoAlert® Mycoplasma Detection Kit?

The MycoAlert® Mycoplasma Detection Assay was designed to “fit” in with normal tissue culturing practices. It was developed in fully supplemented culture media containing antibiotics and DOES NOT require a period of culture without antibiotics...
Missing: stable lines
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