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Do we have any Diseased Human Bronchial/Tracheal Epithelial
Cell
s (DHBE) screened for Air Liquid Interface (ALI) differentiation using the B-ALI™ BulletKit™?
Yes. All our DHBEs are also offered in a version screened for ALI differentiation. The lots which do pass this additional ALI testing are electronically designated by adding an “S” to the end of the original catalog number.
Missing:
stable
lines
What
cell
number do I need per well when using the 96-well Shuttle® Device or the 4D-Nucleocuvette® Stripes?
The
cell
number is very much dependent on the
cell
type you use. For high throughput Nucleofection®, we usually start with one fifth of the optimal amount recommended for the standard Nucleofection® in the 100µl cuvette. Currently the absolute...
Missing:
stable
lines
Do you know if treating
cell
s with 10mM MgCl2 would interfere with the ToxiLight™ Non-Destructive Cytotoxicity BioAssay?
Magnesium (Mg) is a cofactor for both luciferase and Adenylate Kinase (AK) and will speed up their reactions, but this amount of MgCl2 wouldn't make a significant difference.
Missing:
stable
lines
In your optimized Nucleofection® Protocols for MDA-MB-231 and MDA-MB-468, you say to culture the
cell
s without CO2. Why?
The media recommended for these
cells
is Leibovitz's L-15 medium which does not contain sodium bicarbonate to act as a buffer for the carbonic acid that would normally form in the presence of CO2. Furthermore, if the
cells
are cultured in Leibovitz...
Missing:
stable
lines
Can I use the ViaLight™ Plus Assay also, if my
cell
s are cultured in 24well plates, 6well plates or 12 well plates?
To do the VialIght™ Plus Assay on larger volume tissue culture ( eg 6 well , 24 well) add the
cell
lysis reagent equal to half the volume in the plate ( ie 100 µl culture use 50 µl
cell
lysis). Wait 10 minutes for the lysis . Then follow...
Missing:
stable
lines
DNA-purity and Nucleofection®: Can low A260:A280 ratios lead to both reduced transfection efficiency and
cell
viability?
Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio. It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
Missing:
stable
lines
How does Lonza perform the
cell
count on the fresh, unprocessed bone marrow? Does Lonza guarantee a minimum viability for the fresh, unprocessed bone marrow?
Lonza use a Coulter Counter to perform the total
cell
count on the fresh, unprocessed bone marrow. Lonza does not perform viability testing on this product, but when shipped overnight, viability is typically around 80%.
Missing:
stable
lines
Are the Rat Aortic Smooth Muscle
Cell
s R-ASM-580 derived from single donor or does each lot contain a pool from several animals?
The R-ASM-580 per lot contain pooled
cells
from 4-5 male Sprague Dawley rats
Missing:
stable
lines
I transfected neurons using the 4D-Nucleofector® Y unit. During analysis I observed areas with low or no
cell
density, even in my no pulse control sample.
Cells
might have been disturbed or ran dry during pipetting steps. Please perform liquid removal and additions well-by-well. Avoid leaving neurons without any liquid coverage (medium or Nucleofector® Solution). To keep a small liquid film...
Missing:
stable
lines
How much sample do you need for accurate results with MycoAlert® and MycoAlert® PLUS Mycoplasma Detection Kits? Do you need
cell
s or just supernatant?
The standard protocol calls for taking 2 ml of culture media, spinning down any
cells
and removing 100 µl of supernatant. Smaller initial aliquots of media may be taken to start with if necessary.
Missing:
stable
lines
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