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306 results sorted by
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Can I take the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to look at the kinetics of
cell
death?
Yes, by taking a sample of the culture media (usually 20µl) into a fresh luminometer plate this can be assayed with the Adenylate Kinase Detection Reagent supplied in the Toxilight kit to look for increased levels of AK over the control. The rest of...
Missing:
stable
lines
Are the human mesenchymal stem
cell
s (PT-2501) more prone to become differentiated down one lineage over the other?
...differentiation if allowed to reach confluence. As such, to maintain
cells
as undifferentiated
cells
, it is critical to subculture
cells
when they are subconfluent....
Missing:
stable
lines
Where are Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA)
cell
s isolated from?
Lonza’s Normal Human Neural Progenitor (NHNP) and Normal Human Astrocytes (NHA)
cells
are primarily isolated from the cerebral cortex (or forebrain from cerebral hemisphere) from fragmented fetal brain tissue (16-20 weeks old).
Missing:
stable
lines
When I transfected my
cell
s, error 8 appeared on the display of the Nucleofector® IIN, IIS or 2b. What is the reason?
...to the incomplete delivery; however, the
cells
can still be plated for analysis. This generally occurs because the conductivity of the solution was outside of accepted limits – this can happen if the media was not completely aspirated before resuspending, too much...
Missing:
stable
lines
I tested my
cell
culture supernatant negative for mycoplasma contamination using the MycoAlert® Mycoplasma Detection Kit or the MycoAlert® PLUS Mycoplasma Detection Kit. But when I tested the same
cell
s using a PCR-based Assay, I obtained a positive result.
It is possible that the PCR is picking up dead mycoplasma whereas the MycoAlert® and MycoAlert® Plus Assay will pick up only viable mycoplasma. It is possible of course that there is contamination in the PCR giving a false positive.
Missing:
stable
lines
How much sample do you need for accurate results with MycoAlert® Assay? Do you need
cell
s or just supernatant?
The standard protocol calls for taking 2 ml of culture media, spinning down any
cells
and removing 100 µl of supernatant as sample.Smaller initial aliquots of media may be taken to start with if necessary.
Missing:
stable
lines
Can I use the ToxiLight™ Non-Destructive Cytotoxicity BioAssay Kit to assess the effect of transfection procedures on the health of my
cell
s?
...Kinase are present in your transfected
cells
culture supernatant indicating that they have been damaged by the transfection procedure. This would impact on your transfection efficiency values...
Missing:
stable
lines
What optimization is necessary to get the Nucleofector® Technology to work?
None. For each
cell
type in our product list we offer a
cell
-type specific solution and thoroughly optimized electrical parameters. These are already pre-programmed in the Nucleofector® Device. Additionally our optimized protocols give you...
Missing:
stable
Do we have any Diseased Human Bronchial/Tracheal Epithelial
Cell
s (DHBE) screened for Air Liquid Interface (ALI) differentiation using the B-ALI™ BulletKit™?
Yes. All our DHBEs are also offered in a version screened for ALI differentiation. The lots which do pass this additional ALI testing are electronically designated by adding an “S” to the end of the original catalog number.
Missing:
stable
lines
What
cell
number do I need per well when using the 96-well Shuttle® Device or the 4D-Nucleocuvette® Stripes?
The
cell
number is very much dependent on the
cell
type you use. For high throughput Nucleofection®, we usually start with one fifth of the optimal amount recommended for the standard Nucleofection® in the 100µl cuvette. Currently the absolute...
Missing:
stable
lines
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