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What type of matrix should your Clonetics™ embryonic rat/mouse neural cells be seeded onto?

For optimal results, poly-D-lysine with laminin should be used, especially for the hippocampus cells.Poly-D-lysine without the laminin or poly-L-lysine could also be used for the rat and mouse neuronal cells.
Missing: stable linesGuideline

Could you extract cDNA from the lysed cells after detection using the ViaLight™ Plus BioAssay?

The lysis of the cells with the Cell Lysis Reagent included in the ViaLight™ Plus Kit should not affect the DNA. Whilst we haven't conducted this experiment ourselves we cannot see any real reason why it should not be possible...
Missing: stable linesGuideline

How long will your Clonetics™ rat DRG's (dorsal root ganglion cells) remain viable in culture?

The cells can remain viable in culture for about 28 days.
Missing: stable linesGuideline

How many mononuclear cells are typically found per milliliter (mL) of whole, unprocessed bone marrow?

Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear cells per milliliter (mL).
Missing: stable linesGuideline

Can Lonza's Human Adipose-Derived Stem Cells (hADSC) (PT-5006) be expanded prior to differentiation?

Lonza's human adipose derived stem cells (ADSC) are guarantee 5 passages prior to differentiation based on our existing data.
Missing: stable linesGuideline

Can Lonza’s HSMM or SkMC cells be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained...
Missing: stable linesGuideline

Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing cells? What is the purpose of hydrocortisone in the culture media?

Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...
Missing: stable linesGuideline

Should any type of extracellular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem Cells)?

That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you...
Missing: stable linesGuideline

Does phenol red in the cell culture medium interfere with the ToxiLight™ Non-Destructive Cytotoxicity BioAssay?

The assay has been developed in the presence of phenol red so all the example data has been conducted in complete media. Phenol red does quench the light signal so if you compared assays in phenol red free media they would have higher RLUs. The...
Missing: stable linesGuideline

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™ Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.
Missing: stable linesGuideline
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