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Where are Lonza’s Ventricular Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Ventricular Normal Human Cardiac Fibroblast (NHCF-V) from left ventricle.
Missing: stable linesGuideline

Where are Lonza’s Atrial Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Atrial Normal Human Cardiac Fibroblast (NHCF-A) from right atrium.
Missing: stable linesGuideline

How long will your Clonetics™ rat DRG's (dorsal root ganglion cells) remain viable in culture?

The cells can remain viable in culture for about 28 days.
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How many mononuclear cells are typically found per milliliter (mL) of whole, unprocessed bone marrow?

Whole, unprocessed bone marrow typically contains 4 million to 10 million mononuclear cells per milliliter (mL).
Missing: stable linesGuideline

Can Lonza's Human Adipose-Derived Stem Cells (hADSC) (PT-5006) be expanded prior to differentiation?

Lonza's human adipose derived stem cells (ADSC) are guarantee 5 passages prior to differentiation based on our existing data.
Missing: stable linesGuideline

Can Lonza’s HSMM or SkMC cells be maintained/subcultured/cryopreserved as myotubes after they have been differentiated?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Differentiated HSMM myotubes can be maintained...
Missing: stable linesGuideline

Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing cells? What is the purpose of hydrocortisone in the culture media?

Hydrocortisone is used for at least two purposes in Lonza’s primary cell culture media. Hydrocortisone (in combination with low serum concentrations) will inhibit the takeover of the culture by fibroblasts which may be present in small numbers from...
Missing: stable linesGuideline

Should any type of extracellular matrix be used when growing undifferentiated hMSC's (human Mesenchymal Stem Cells)?

That will depend on the type of medium used. If you are using our MSCGM™ BulletKit™ PT-3001, we do not recommend using a matrix with the cells as it might induce differentiation and make the cells difficult to detach from the culture vessel. If you...
Missing: stable linesGuideline

In Air-Liquid-Interface (ALI) Culture, won't the apical surface of B-ALI™ Cells dry out?

No, the cells secrete fluid at the apical side but also moisture is drawn through the cell layer from the basal layer.
Missing: stable linesGuideline

How can I remove the cells from the 100 uL Nucleocuvette® Vessel after Nucleofection®? Is there any alternative?

We recommend using the plastic single use pipettes provided in the kits. These pipettes prevent any damage to the cells and ensure a good cell recovery.
Missing: stable linesGuideline
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