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Will Lonza’s HSMM cells de-differentiate after they have been differentiated to form myotubes?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Additionally, differentiation...
Missing: stable linesGuideline

What species of rats and mice are your Clonetics™ embryonic neuronal cells isolated from?

The rats are Sprague-Dawley and the mice are CD1 or C57.
Missing: stable linesGuideline

Where are Lonza’s Human Skeletal Muscle Myoblasts (HSMM) cells isolated from?

The Human Skeletal Muscle Myoblasts are sourced from normal post-gestational tissue of cadavers from 1 day to 40 years old. The HSMMs are isolated from the quadriceps or psoas muscle.
Missing: stable linesGuideline

Where are Lonza’s Ventricular Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Ventricular Normal Human Cardiac Fibroblast (NHCF-V) from left ventricle.
Missing: stable linesGuideline

Where are Lonza’s Atrial Normal Human Cardiac Fibroblast cells isolated from?

Lonza obtains our Atrial Normal Human Cardiac Fibroblast (NHCF-A) from right atrium.
Missing: stable linesGuideline

How many cells do I have to use for each well of the 16-well Nucleocuvette® Strip?

This depends on the cell type you are working with. In our Optimized Protocols the cell numbers range from 2.5 x 10^4 cells (mouse DC) up to 1x 10^6 cells (human T cells). Detailed information is provided in our 4D-Nucleofector® Optimized Protocols.
Missing: stable linesGuideline

When would be the optimal time to perform experiments post osteogenic differentiation for human mesenchymal stem cells?

Once the cells have differentiated and started laying down bone matrix, we recommend assaying the cells immediately. If cells begin to delaminate (peel of the culture plate in layers), this would indicate that the cells are embedded in the calcium...
Missing: stable linesGuideline

Which cell numbers should I use when running the ToxiLight™ Non-Destructive Cytotoxicity BioAssay

Toxilight works much better at low cell numbers, for adherent cell lines < 10,000 in one well of a 96well plate and < 50,000 for suspension.We would normally seed 10,000 cells/well when using adherent cells to allow enough room in the well...
Missing: stable linesGuideline

Are there any substitutions that can be used in place of the HSA in the freezing media for hMSC's (Human Mesenchymal Stem Cells)?

Cryopreservation may compromise cell quality and performance. Lonza CANNOT guarantee performance of Clonetics™ & Poietics™ Cells that have been cryopreserved outside of Lonza. To avoid loss of cells and forfeiture of your warranty, we recommend...
Missing: stable linesGuideline

Do you recommend a purification method to purify specific blood/immune cell populations before Nucleofection®?

We recommend using negative selection (depletion) because your purified cells are "untouched", not influenced, and strictly not activated.Positive selection may cause increasing amounts of dead cells and could also lead to activation of the cells...
Missing: stable linesGuideline
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