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306 results sorted by
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What is the recommended culturing protocol for Lonza's bone marrow stromal
cell
s (2M-302)?
Lonza's bone marrow stromal
cells
should be thawed according to Lonza's "Procedure for thawing Poietics™
Cells
". Upon thaw,
cells
should be plated at 3,000-4,000
cells
per cm² in MyeloCult™ H5100 from Stem
Cell
Technologies, Inc. An adherent...
Missing:
stable
lines
What plate format is the protocol for culturing Lonza's Osteoclast Precursor (OCP)
cell
s written for?
The protocol for culturing Lonza's Osteoclast Precursor (OCP)
cells
written for plating
cells
into a 96-well plate.
Missing:
stable
lines
For cultivation of Smooth Muscle
Cell
s - Can the insulin be left out of the SmGM™-2 Media?
Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the
cells
for long term growth (ie. 4 - 5 passages), however smooth muscle
cells
do survive quite well short term without insulin.Insulin...
Missing:
stable
lines
What is the effect of
cell
ular ATPases on the results obtained with the ViaLight™ Plus
Cell
Proliferation and Cytotoxicity BioAssay?
The ViaLight™ Plus
Cell
Lysis Reagent inhibits ATPase activity - mainly by using a low pH and denaturing the cellular proteins. This is why the ViaLight™ Plus Assay has such a good half life. So
cell
expressing high ATPase will probably...
Missing:
stable
lines
Why does Lonza recommend using non-ventilated caps when culturing insect
cell
s?
The reason is that insect
cells
are often incubated in very simple incubators that are not humidified (and without CO2 at 24°C to 28°C). Insect media typically do not need CO2 for buffering pH, so gas exchange is not required. To avoid evaporation...
Missing:
stable
lines
Will Lonza’s HSMM
cell
s de-differentiate after they have been differentiated to form myotubes?
The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these
cells
will not de-differentiate to myoblasts. Additionally, differentiation...
Missing:
stable
lines
What species of rats and mice are your Clonetics™ embryonic neuronal
cell
s isolated from?
The rats are Sprague-Dawley and the mice are CD1 or C57.
Missing:
stable
lines
Where are Lonza’s Human Skeletal Muscle Myoblasts (HSMM)
cell
s isolated from?
The Human Skeletal Muscle Myoblasts are sourced from normal post-gestational tissue of cadavers from 1 day to 40 years old. The HSMMs are isolated from the quadriceps or psoas muscle.
Missing:
stable
lines
Where are Lonza’s Ventricular Normal Human Cardiac Fibroblast
cell
s isolated from?
Lonza obtains our Ventricular Normal Human Cardiac Fibroblast (NHCF-V) from left ventricle.
Missing:
stable
lines
Where are Lonza’s Atrial Normal Human Cardiac Fibroblast
cell
s isolated from?
Lonza obtains our Atrial Normal Human Cardiac Fibroblast (NHCF-A) from right atrium.
Missing:
stable
lines
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