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What are Lonza's human Mesenchymal Stem Cells (PT-2501) cryopreserved in?

The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)
Missing: stable linesGuideline

Where are Lonza’s Normal Human Osteoblasts (NHOst) cells isolated from?

Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
Missing: stable linesGuideline

Can I use a medium other than the one you offer to culture Clonetics® Cells?

We have an optimized medium for each Clonetics® Cell type. The cells are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics® cells only if appropriate Clonetics® media and reagents are used...
Missing: stable linesGuideline

What cell numbers can I transfect when using the 4D-Nucleofector® System?

The 4D-Nucleofector® System offers great flexibility in terms of cell numbers.Using the 4D-Nucleofector® X Unit it is possible to perform Nucleofection® in 100 µl and 20 µl Format.The 100 µl Nucleocuvette® Format is suited for high cell...
Missing: stable linesGuideline

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
Missing: stable linesGuideline

What plate format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?

The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for plating cells into a 96-well plate.
Missing: stable linesGuideline

For cultivation of Smooth Muscle Cells - Can the insulin be left out of the SmGM™-2 Media?

Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin...
Missing: stable linesGuideline

What is the effect of cellular ATPases on the results obtained with the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay?

The ViaLight™ Plus Cell Lysis Reagent inhibits ATPase activity - mainly by using a low pH and denaturing the cellular proteins. This is why the ViaLight™ Plus Assay has such a good half life. So cell expressing high ATPase will probably...
Missing: stable linesGuideline

Why does Lonza recommend using non-ventilated caps when culturing insect cells?

The reason is that insect cells are often incubated in very simple incubators that are not humidified (and without CO2 at 24°C to 28°C). Insect media typically do not need CO2 for buffering pH, so gas exchange is not required. To avoid evaporation...
Missing: stable linesGuideline

Will Lonza’s HSMM cells de-differentiate after they have been differentiated to form myotubes?

The differentiation of Lonza’s Human Skeletal Muscle Myoblasts (HSMM) to myotubes is a terminal differentiation and, therefore, once differentiated, these cells will not de-differentiate to myoblasts. Additionally, differentiation...
Missing: stable linesGuideline
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