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Where are Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) isolated from?

While Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) are isolated from either the ascending or descending aorta, it is usually the ascending aorta that is used. If the heart comes with the aorta, which happens most of the time, then the AoSMC...
Missing: stable linesGuideline

What testing methods are currently used to validate differentiation of Lonza's mesenchymal stem cells?

Lonza currently validates trilineage differentiation of human mesenchymal stem cells using the following quantitative assays:Adipogenic Differentiation: lipid vacuole binding with AdipoRed (catalog no. PT-7009)Osteogenic Differentiation: inorganic...
Missing: stable linesGuideline

Are there any publications comparing Lonza's hepatocytes to hepatocytes derived from pluripotent stem cells? 

There is a citation from King's College London comparing Lonza's primary hepatocytes to stem-cell derived hepatocytes. Here is the link: https://sciencematters.io/articles/202004000002 Here are few key points from the article: CYP1A2...
Missing: stable linesGuideline

Can I use a medium other than the one you offer to culture Clonetics® Cells?

We have an optimized medium for each Clonetics® Cell type. The cells are grown and tested in their specific medium system. Lonza guarantees the performance of Clonetics® cells only if appropriate Clonetics® media and reagents are used...
Missing: stable linesGuideline

What is the recommended culturing protocol for Lonza's bone marrow stromal cells (2M-302)?

Lonza's bone marrow stromal cells should be thawed according to Lonza's "Procedure for thawing Poietics™ Cells". Upon thaw, cells should be plated at 3,000-4,000 cells per cm² in MyeloCult™ H5100 from StemCell Technologies, Inc. An adherent...
Missing: stable linesGuideline

What cell numbers can I transfect when using the 4D-Nucleofector® System?

The 4D-Nucleofector® System offers great flexibility in terms of cell numbers.Using the 4D-Nucleofector® X Unit it is possible to perform Nucleofection® in 100 µl and 20 µl Format.The 100 µl Nucleocuvette® Format is suited for high cell...
Missing: stable linesGuideline

What plate format is the protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for?

The protocol for culturing Lonza's Osteoclast Precursor (OCP) cells written for plating cells into a 96-well plate.
Missing: stable linesGuideline

For cultivation of Smooth Muscle Cells - Can the insulin be left out of the SmGM™-2 Media?

Yes, insulin can be left out of the SmGM™-2 media for as long as 1-3 passages.Insulin is beneficial to the cells for long term growth (ie. 4 - 5 passages), however smooth muscle cells do survive quite well short term without insulin.Insulin...
Missing: stable linesGuideline

What is the effect of cellular ATPases on the results obtained with the ViaLight™ Plus Cell Proliferation and Cytotoxicity BioAssay?

The ViaLight™ Plus Cell Lysis Reagent inhibits ATPase activity - mainly by using a low pH and denaturing the cellular proteins. This is why the ViaLight™ Plus Assay has such a good half life. So cell expressing high ATPase will probably...
Missing: stable linesGuideline

Why does Lonza recommend using non-ventilated caps when culturing insect cells?

The reason is that insect cells are often incubated in very simple incubators that are not humidified (and without CO2 at 24°C to 28°C). Insect media typically do not need CO2 for buffering pH, so gas exchange is not required. To avoid evaporation...
Missing: stable linesGuideline
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