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Can I expand the mononuclear cells, CD34+ progenitors, without differentiating them?

No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.
Missing: stable linesGuideline

What passage number are the Clonetics™ mouse and rat embryonic neuronal cells cryopreserved at?

The cells are cryopreserved at passage 0.
Missing: stable linesGuideline

Where are Lonza’s lung microvascular endothelial cells (HMVEC-L) isolated from?

Lonza’s lung microvascular endothelial cells (HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing: stable linesGuideline

Can cell free supernatants ( NOT lysed) be stored for later testing with ViaLight™ Plus BioAssay?

For supernatants there will be exogenous ATPases present that will degrade ATP so it should really be treated as if it were cells. Freeze as quickly as possible and store at -80C, for no more than 4 weeks. The ATP will be OK with the freezing.
Missing: stable linesGuideline

When performing the MycoAlert® Mycoplasma Detection Assay, why is it necessary to spin out the cells?

It is highly recommended that the samples are spun down prior to testing. If cells are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background...
Missing: stable linesGuideline

I want to stimulate human primary T-cells with anti-CD3 and anti-CD28. Do you have a detailed protocol for this?

Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T Cells.For optimal stimulation of the transfected cells we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
Missing: stable linesGuideline

Where are Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) isolated from?

While Lonza’s Human Aortic Smooth Muscle Cells (AoSMC) are isolated from either the ascending or descending aorta, it is usually the ascending aorta that is used. If the heart comes with the aorta, which happens most of the time, then the AoSMC...
Missing: stable linesGuideline

What testing methods are currently used to validate differentiation of Lonza's mesenchymal stem cells?

Lonza currently validates trilineage differentiation of human mesenchymal stem cells using the following quantitative assays:Adipogenic Differentiation: lipid vacuole binding with AdipoRed (catalog no. PT-7009)Osteogenic Differentiation: inorganic...
Missing: stable linesGuideline

Are there any publications comparing Lonza's hepatocytes to hepatocytes derived from pluripotent stem cells? 

There is a citation from King's College London comparing Lonza's primary hepatocytes to stem-cell derived hepatocytes. Here is the link: https://sciencematters.io/articles/202004000002 Here are few key points from the article: CYP1A2...
Missing: stable linesGuideline

What is the age of the rats and mice the Clonetics™ embryonic neuronal cells are isolated from?

Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.
Missing: stable linesGuideline
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