Data Type
Cell Information
(508)
Citations
(4782)
FAQ
(313)
Culture Media
(45)
Category
Primary Cells and Media
(161)
Transfection
(105)
Bioassay
(31)
Mycoplasma Detection and Prevention
(16)
Laboratory Instrumentation
(14)
Endotoxin and Pyrogen Testing
(12)
Serum-free and Speciality Media
(11)
Cell Lines and Primary Cancer Cells
(7)
Classical Media and Reagents
(6)
3D Cell Culture
(4)
Cell Services
(3)
Bioprocess Containers
(1)
Live Cell Imaging
(1)
+ Show All
Research Area
Basic Research
(100)
Cancer Research/Cell Biology
(67)
Immunotherapy / Hematology
(54)
Gene Expression
(38)
Stem Cells
(34)
Respiratory Research
(26)
Cardiovascular
(21)
Neurobiology
(18)
Regenerative medicine
(18)
Uncategorized
(18)
Toxicology
(16)
Dermatology/Tissue Engineering
(8)
Drug Discovery
(6)
Molecular Biology
(5)
Endotoxin Testing
(3)
Gastroenterology
(1)
Parasitology
(1)
+ Show All
313 results sorted by
relevance
alphabetical
newest first
oldest first
Do I need a special media or protocol for culturing the Clonetics™ and Poietics™ Diseased
Cell
s?
No, all of our diseased
cells
are isolated, expanded, and tested in the same media and using the same protocol as the normal counterpart
cells
.
Missing:
stable
linesGuideline
Can I expand the mononuclear
cell
s, CD34+ progenitors, without differentiating them?
No, the
cells
will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the
cells
.
Missing:
stable
linesGuideline
What passage number are the Clonetics™ mouse and rat embryonic neuronal
cell
s cryopreserved at?
The
cells
are cryopreserved at passage 0.
Missing:
stable
linesGuideline
What is the age of the rats and mice the Clonetics™ embryonic neuronal
cell
s are isolated from?
Both are of embryonic origin: the rats are E18 or E19 and the mice are E14 or E15.
Missing:
stable
linesGuideline
What are Lonza's human Mesenchymal Stem
Cell
s (PT-2501) cryopreserved in?
The cryopreservation medium is: 85% MSCBM 10% DMSO 5% Human Serum Albumin (w/v)
Missing:
stable
linesGuideline
Where are Lonza’s Normal Human Osteoblasts (NHOst)
cell
s isolated from?
Lonza’s Normal Human Osteoblasts (NHOst) are isolated by explant, and the tissue is from the spongy section of the bone head of the ribs, femur, or tibia.
Missing:
stable
linesGuideline
Where are Lonza’s lung microvascular endothelial
cell
s (HMVEC-L) isolated from?
Lonza’s lung microvascular endothelial
cells
(HMVEC-L) are isolated from the tissue directly beneath the outer lining of the lung. All vessels isolated are smaller than can be seen with the naked eye.
Missing:
stable
linesGuideline
Can
cell
free supernatants ( NOT lysed) be stored for later testing with ViaLight™ Plus BioAssay?
For supernatants there will be exogenous ATPases present that will degrade ATP so it should really be treated as if it were
cells
. Freeze as quickly as possible and store at -80C, for no more than 4 weeks. The ATP will be OK with the freezing.
Missing:
stable
linesGuideline
When performing the MycoAlert® Mycoplasma Detection Assay, why is it necessary to spin out the
cell
s?
It is highly recommended that the samples are spun down prior to testing. If
cells
are present in the assay they will contribute to the initial reading producing a higher background. This in turn can decrease the difference between the background...
Missing:
stable
linesGuideline
I want to stimulate human primary T-
cell
s with anti-CD3 and anti-CD28. Do you have a detailed protocol for this?
Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T
Cells
.For optimal stimulation of the transfected
cells
we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
Missing:
stable
linesGuideline
PREVIOUS
PAGE
11
PAGE
12
PAGE
13
PAGE
14
PAGE
15
PAGE
16
PAGE
17
PAGE
18
PAGE
19
NEXT