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307 results sorted by
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What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem
cell
s?
TGF-B3 has worked best in our hands so that is what we recommend.
Missing:
stable
lines
What is the primary differentiation reagent for chondrogenic differentiation of mesenchymal stem
cell
s?
Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
Missing:
stable
lines
Does the basal media portion of the TheraPEAK™ Mesenchymal Stem
Cell
Growth Medium (TheraPeak™ MSCGM™) contain proteins?
The basal media portion of the TheraPEAK™ Mesenchymal Stem
Cell
Growth Medium (MSCGM™), catalog no BEBP12-934Q, contains recombinant and human serum derived proteins.
Missing:
stable
lines
How many different primary
cell
kits are available for the 4D-Nucleofector® System?
In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary
cell
type, the 4D-Nucleofector® System only requires 5 primary
cell
kits covering a broad range of primary
cells
. There are
cell
...
Missing:
stable
lines
Does your Nucleofector® Solutions contain anything that would inhibit attachment of adherent
cell
s?
No. In many cases where decreased attachment is a problem, the cause is inactivated trypsin. Unless the trypsin is inactivated with trypsin inhibitor or media containg BSA or serum, it will continue to degrade the
cells
and ultimately decrease
cell
...
Missing:
stable
lines
Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's
cell
products?
Lonza does obtain the Human Leukocyte Antigen (HLA) type information for many (but not all) of the Clonetics® and Poietics®
Cell
Types we are offering. If you require
cells
of a specific HLA type donor, please contact Lonza's Scientific Support Team...
Missing:
stable
lines
Do I need a special media or protocol for culturing the Clonetics™ and Poietics™ Diseased
Cell
s?
No, all of our diseased
cells
are isolated, expanded, and tested in the same media and using the same protocol as the normal counterpart
cells
.
Missing:
stable
lines
Can I expand the mononuclear
cell
s, CD34+ progenitors, without differentiating them?
No, the
cells
will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the
cells
.
Missing:
stable
lines
What passage number are the Clonetics™ mouse and rat embryonic neuronal
cell
s cryopreserved at?
The
cells
are cryopreserved at passage 0.
Missing:
stable
lines
Can
cell
free supernatants ( NOT lysed) be stored for later testing with ViaLight™ Plus BioAssay?
For supernatants there will be exogenous ATPases present that will degrade ATP so it should really be treated as if it were
cells
. Freeze as quickly as possible and store at -80C, for no more than 4 weeks. The ATP will be OK with the freezing.
Missing:
stable
lines
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