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What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem cells?

TGF-B3 has worked best in our hands so that is what we recommend.
Missing: stable lines

What is the primary differentiation reagent for chondrogenic differentiation of mesenchymal stem cells?

Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
Missing: stable lines

Where are Lonza’s NHOst CC-2538 cells isolated from?

Lonza obtains our Normal Human Osteoblasts (NHOst) from the spongy section of the bone head of ribs, femur, radius, fibula or tibia.If you would like to know, from which specific bone your lot of NHOst has been isolated, just contact our Scientific...
Missing: stable lines

I want to stimulate human primary T-cells with anti-CD3 and anti-CD28. Do you have a detailed protocol for this?

Yes, the detailed protocol is part of our 4D-Nucleofector® Protocol for stimulated Human T Cells.For optimal stimulation of the transfected cells we recommend coating 96-wells plates for stimulation [Nunc Immuno™ Plate C96 Maxi Sorp™, Cat. No. 430...
Missing: stable lines

How many different primary cell kits are available for the 4D-Nucleofector® System?

In contrast to the Nucleofector® I, II and 2b System which comes with individual kits for each primary cell type, the 4D-Nucleofector® System only requires 5 primary cell kits covering a broad range of primary cells. There are cell...
Missing: stable lines

Does your Nucleofector® Solutions contain anything that would inhibit attachment of adherent cells?

No. In many cases where decreased attachment is a problem, the cause is inactivated trypsin. Unless the trypsin is inactivated with trypsin inhibitor or media containg BSA or serum, it will continue to degrade the cells and ultimately decrease cell...
Missing: stable lines

Is the Human Leukocyte Antigen (HLA) type information available for any of Lonza's cell products?

Lonza does obtain the Human Leukocyte Antigen (HLA) type information for many (but not all) of the Clonetics® and Poietics® Cell Types we are offering. If you require cells of a specific HLA type donor, please contact Lonza's Scientific Support Team...
Missing: stable lines

Do I need a special media or protocol for culturing the Clonetics™ and Poietics™ Diseased Cells?

No, all of our diseased cells are isolated, expanded, and tested in the same media and using the same protocol as the normal counterpart cells.
Missing: stable lines

Can I expand the mononuclear cells, CD34+ progenitors, without differentiating them?

No, the cells will proliferate and differentiate simultaneously upon being placed in culture. Varying the cytokine cocktail can influence the differentiation and proliferation of the cells.
Missing: stable lines

What passage number are the Clonetics™ mouse and rat embryonic neuronal cells cryopreserved at?

The cells are cryopreserved at passage 0.
Missing: stable lines
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