Data Type

Category

+ Show All

Research Area

+ Show All
306 results sorted by

Are the Clonetics™ embryonic rat/mouse neuronal cells from a single donor or pooled?

The cells are pooled from whole litters, including both males and females.
Missing: stable lines

Do you have any instructions available for using a hemocytometer for cell counting?

Yes. Our technical guideline "Cell Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.
Missing: stable lines

Are Lonza’s human mammary epithelial cells tested for estrogen receptor response?

Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial cells being isolated in the HMEC product.
Missing: stable lines

What concentration of Trypsin/EDTA do you recommend for subculturing primary cells?

For most cell types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.
Missing: stable lines

What medium and which cytokines are recommended for Natural Killer (NK) cell culture?

For the cultivation of Natural Killer (NK) cells we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.
Missing: stable lines

How many mycoplasma species exist? Which are the usual suspects in cell culture?

More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from cell culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale...
Missing: stable lines

What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem cells?

TGF-B3 has worked best in our hands so that is what we recommend.
Missing: stable lines

What is the primary differentiation reagent for chondrogenic differentiation of mesenchymal stem cells?

Lonza recommends the addition of TGF-B3 (PT-4124) to the Chondrogenic Differentiation Media (PT-3003) to promote chondrogenic differentiation of MSCs. Please see the MSC differenation protocol for complete details on differentiating MSCs down a...
Missing: stable lines

Where are Lonza’s NHOst CC-2538 cells isolated from?

Lonza obtains our Normal Human Osteoblasts (NHOst) from the spongy section of the bone head of ribs, femur, radius, fibula or tibia.If you would like to know, from which specific bone your lot of NHOst has been isolated, just contact our Scientific...
Missing: stable lines

What is the shelf-life for all the media associated with Human mesenchymal stem cells?

The shelf-life of the MSCGM™ Mesenchymal Stem Cell Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...
Missing: stable lines
PAGE 14

Privacy | Legal | About Lonza

© Lonza. All rights reserved.