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(16)
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320 results sorted by
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alphabetical
newest first
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Can I store my cryopreserved primary
cell
ampule at –80°C?
No, cryopreserved
cells
should be stored in liquid or vapor phase nitrogen. Storing the
cells
at higher temperatures can irreversibly damage the
cells
.
Missing:
stable
lines
What is the component labeled "MCGS" in the mesenchymal stem
cell
growth and differentiation media?
Mesenchymal
Cell
Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem
cells
PT-2501
Missing:
stable
lines
Are the Clonetics® embryonic rat/mouse neuronal
cell
s from a single donor or pooled?
The
cells
are pooled from whole litters, including both males and females.
Missing:
stable
lines
Do you have any instructions available for using a hemocytometer for
cell
counting?
Yes. Our technical guideline "
Cell
Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below..
Missing:
stable
lines
Are Lonza’s human mammary epithelial
cell
s tested for estrogen receptor response?
Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial
cells
being isolated in the HMEC product.
Missing:
stable
lines
What concentration of Trypsin/EDTA do you recommend for subculturing primary
cell
s?
For most
cell
types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.
Missing:
stable
lines
What medium and which cytokines are recommended for Natural Killer (NK)
cell
culture?
For the cultivation of Natural Killer (NK)
cells
we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.
Missing:
stable
lines
How many mycoplasma species exist? Which are the usual suspects in
cell
culture?
More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from
cell
culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale...
Missing:
stable
lines
What is the shelf-life for all the media associated with Human mesenchymal stem
cell
s?
The shelf-life of the MSCGM™ Mesenchymal Stem
Cell
Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...
Missing:
stable
lines
What growth factor is pertinent in chondrogenic differentiation of mesenchymal stem
cell
s?
TGF-B3 has worked best in our hands so that is what we recommend.
Missing:
stable
lines
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