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306 results sorted by
relevance
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newest first
oldest first
Are the Clonetics™ embryonic rat/mouse neuronal
cell
s from a single donor or pooled?
The
cells
are pooled from whole litters, including both males and females.
Missing:
stable
lines
Do you have any instructions available for using a hemocytometer for
cell
counting?
Yes. Our technical guideline "
Cell
Counting and Determination of Viability via Hemocytometer" can be found as associated pdf file in the link below.
Missing:
stable
lines
Are Lonza’s human mammary epithelial
cell
s tested for estrogen receptor response?
Unfortunately, Lonza has not thoroughly investigated the estrogen receptor response/response to estrogen of the mammary epithelial
cells
being isolated in the HMEC product.
Missing:
stable
lines
What concentration of Trypsin/EDTA do you recommend for subculturing primary
cell
s?
For most
cell
types, Lonza recommends the Trypsin/EDTA provided as one of the components in our ReagentPack™ Subculturing Reagents Kit (CC-5034).The concentration of this solution is proprietary.
Missing:
stable
lines
What medium and which cytokines are recommended for Natural Killer (NK)
cell
culture?
For the cultivation of Natural Killer (NK)
cells
we recommend using the X-VIVO 15 medium.In regard to cytokines, this varies depending upon the application, but many cited publications indicate using IL-2 for activation and longer term culture.
Missing:
stable
lines
How many mycoplasma species exist? Which are the usual suspects in
cell
culture?
More than 180 species have been identified of which 20 distinct Mycoplasma and Acholeplasma species from human, bovine and swine have been isolated from
cell
culture. There are 6 species that account for 95% of all mycoplasma infections (M.orale...
Missing:
stable
lines
What is the shelf-life for all the media associated with Human mesenchymal stem
cell
s?
The shelf-life of the MSCGM™ Mesenchymal Stem
Cell
Growth Medium BulletKit™ (PT-3001) once the SingleQuots Kit is added is 30 days. For the differentiation kits:hMSC differentiation BulletKit™ – osteogenic (PT-3002)hMSC differentiation...
Missing:
stable
lines
What additional donor information is available for Lonza’s diseased airway
cell
s?
In addition to the standardly available donor information (age, gender, race), Lonza can provide the following additional information for most lots of our diseased airway
cells
: - smoking history (including volume and duration), - any medications...
Missing:
stable
lines
How old are the donors for Lonza’s dermal
cell
s from neonatal donors?
The neonatal foreskin tissue used to isolate Lonza’s dermal
cells
from neonatal donors is almost always collected within the first few days after birth, and at most a week or two after birth. At most U.S. hospitals, circumcisions are performed...
Missing:
stable
lines
Does the basal media portion of the TheraPEAK™ Mesenchymal Stem
Cell
Growth Medium (TheraPeak™ MSCGM™) contain proteins?
The basal media portion of the TheraPEAK™ Mesenchymal Stem
Cell
Growth Medium (MSCGM™), catalog no BEBP12-934Q, contains recombinant and human serum derived proteins.
Missing:
stable
lines
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