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Where are Lonza’s NHAC-kn cells isolated from?

Lonza obtains our Normal Human Articular Chondrocytes (NHAC-kn) from the weight bearing cartilage of the knee (hyaline cartilage)
Missing: stable lines

What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.
Missing: stable lines

Can you use the AdipoRed™ Assay on cells other than preadipocytes?

Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.
Missing: stable lines

Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing cells? What is the purpose of hydrocortisone in the culture media?

...the original cell isolation. While the hydrocortisone will not complete kill or prevent the growth of the contaminating fibroblasts, it will slow the proliferation down so that the other cell line may proliferate and dominate. Hydrocortisone will also help...
Missing: stable

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...
Missing: stable lines

Will Lonza’s diseased airway cells retain their diseased phenotype throughout culturing?

With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium...
Missing: stable lines

Can I store my cryopreserved primary cell ampule at –80°C?

No, cryopreserved cells should be stored in liquid or vapor phase nitrogen. Storing the cells at higher temperatures can irreversibly damage the cells.
Missing: stable lines

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells...
Missing: stable lines

Co-culture protocol for Hepatocytes and Non-Parenchymal Cells/NPC (2D) 

If you are searching for a 2D co-culture protocol for Hepatocytes and Non-Parenchymal Cells (NPC), please find our co-culture protocol on Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-CultureAnd in case you want to work high-throughput, here...
Missing: stable lines

What is the component labeled "MCGS" in the mesenchymal stem cell growth and differentiation media?

Mesenchymal Cell Growth Supplement (MCGS) is 100% fetal bovine serum prescreened to neither induce nor prevent differentiation of human bone marrow derived stem cells PT-2501
Missing: stable lines
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