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Can MycoZap™ Prophlyactic be used in cell cultures expressing a Tet repressor?

One of the MycoZap™ Prophylactic components interacts with the Tet repressor, leading to unwanted expression of genes expressed under the control of a Tet response element in lines. Therefore, MycoZap™ Prophylactic is not suited for cell cultures...
Missing: stable linesGuideline

What are the critical steps for successful Nucleofection® of monocytic cell lines like THP-1, HL60 and U-937?

To achieve optimal results, we strongly recommend following the Optimized Protocols in regard to cell culture conditions (medium, splitting cycle, density before Nucleofection), DNA amount and purity.Please also be sure not to exceed 90xg when...
Missing: stable linesGuideline

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...
Missing: stable linesGuideline

Do the Clonetics™ rat and mouse embryonic neuronal cells proliferate?

No, the neurons do not proliferate.
Missing: stable linesGuideline

How can I detect mycoplasma contamination of my cells?

We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid...
Missing: stable linesGuideline

Does the recommended media for each of the diabetic cells contain glucose?

The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations. 
Missing: stable linesGuideline

What is the concentration of sugar in Lonza’s endothelial cell media?

While the complete formulation of all of Lonza’s specialty media are proprietary, the EBM™, EBM™-2, and EBM™ phenol red free basal media will all contain D-glucose (dextrose) [CAS number 50-99-7] at exactly 1.0 g/L (5.55 mM). There are no other...
Missing: stable linesGuideline

Where are Lonza’s NHAC-kn cells isolated from?

Lonza obtains our Normal Human Articular Chondrocytes (NHAC-kn) from the weight bearing cartilage of the knee (hyaline cartilage)
Missing: stable linesGuideline

Will Lonza’s diseased airway cells retain their diseased phenotype throughout culturing?

With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium...
Missing: stable linesGuideline

What is the percentage of purity of Lonza’s Normal Human Osteoblasts (NHOst) cells?

It is not easy to put an exact number to the purity of the NHOst populations because the tests used are all functional assays: alkaline phosphatase production and bone mineralization, which unless you have synchronized cultures, not all the cells...
Missing: stable linesGuideline
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