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Where are Lonza’s NHAC-kn cells isolated from?

Lonza obtains our Normal Human Articular Chondrocytes (NHAC-kn) from the weight bearing cartilage of the knee (hyaline cartilage)
Missing: stable linesGuideline

Where are Lonza’s Bronchial/Tracheal Epithelial cells isolated from?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
Missing: stable linesGuideline

How is the disease state for Lonza’s diseased airway cells determined?

The tissue received for all of Lonza’s diseased airway cells are obtained from donors which were diagnosed by an accredited physician prior to death. Frequently, Lonza will obtain information on medications taken related to the disease which...
Missing: stable linesGuideline

Where are Lonza’s Small Airway Epithelial cells isolated from?

Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...
Missing: stable linesGuideline

What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.
Missing: stable linesGuideline

Can you use the AdipoRed™ Assay on cells other than preadipocytes?

Yes, the AdipoRed™ Assay Reagent can be used on many other cell types that accumulate triglycerides. It may have to be optimized for specific applications depending on the lipid that is stained.
Missing: stable linesGuideline

Can MycoZap™ Prophlyactic be used in cell cultures expressing a Tet repressor?

One of the MycoZap™ Prophylactic components interacts with the Tet repressor, leading to unwanted expression of genes expressed under the control of a Tet response element in lines. Therefore, MycoZap™ Prophylactic is not suited for cell cultures...
Missing: stable linesGuideline

Where are Lonza's normal Adipose Derived Stem Cells isolated from?

Lonza's normal (non-diabetic) Adipose Derived Stem Cells (ADSCs) are isolated from lipoaspirate obtained from liposuction surgeries. As liposuction surgeries typically pool lipoaspirate from multiple locations, a single isolation source is not able...
Missing: stable linesGuideline

What are the critical steps for successful Nucleofection® of monocytic cell lines like THP-1, HL60 and U-937?

To achieve optimal results, we strongly recommend following the Optimized Protocols in regard to cell culture conditions (medium, splitting cycle, density before Nucleofection), DNA amount and purity.Please also be sure not to exceed 90xg when...
Missing: stable linesGuideline

Will Lonza’s diseased airway cells retain their diseased phenotype throughout culturing?

With limited exceptions, Lonza does not test the diseased cells as part of our standard Quality Control testing in a manner which would validate the disease diagnosis. All of Lonza’s diseased airway cells are cultured using the same medium...
Missing: stable linesGuideline
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