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What primary human diseased airway cells does Lonza offer?

Lonza's current portfolio for human diseased airway cells includes:- Bronchial Epithelial Cells- Lung Fibroblasts- Bronchial Smooth Muscle Cells...from asthmatic and COPD donors and if available from donors with Cystic fibrosis.An overview...
Missing: stable lines

Which promoter can be recommended for transient gene expression in mammalian cells ?

Vectors with a CMV promoter typically work fine for transient protein expression in mammalian cells, like HEK293, CHO or NSO. For insect cells, like Sf9 or Sf21, vectors with insect promoters are required. In HEK293E (EBNA) cells, the use...
Missing: stable lines

How many passages can I use the Clonetics™ diseased cells?

Proliferative capacity of Lonza's Clonetics™ diseased cells is tested through one or more passages (depending on the cell type) for information only.It is NOT a specification where a minimum number of doublings must be obtained in order to pass...
Missing: stable lines

What kind of effects could a mycoplasma contamination have on my cells?

Mycoplasma contamination can: > Inhibit cell metabolism > Cause cell death > Induce morphological alterations > Alter DNA, RNA, and protein synthesis > Induce chromosomal aberrations > Cause increased sensitivity to inducers...
Missing: stable lines

How is the disease state for Lonza’s diseased airway cells determined?

The tissue received for all of Lonza’s diseased airway cells are obtained from donors which were diagnosed by an accredited physician prior to death. Frequently, Lonza will obtain information on medications taken related to the disease which...
Missing: stable lines

Where are Lonza’s Small Airway Epithelial cells isolated from?

Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...
Missing: stable lines

How can I detect mycoplasma contamination of my cells?

We recommend using the 20-minute MycoAlert® Mycoplasma Detection Kit from Lonza. The simple four-step MycoAlert® Assay is a selective biochemical test that exploits the activity of mycoplasmal enzymes. The presence of these enzymes provides a rapid...
Missing: stable lines

Do the Clonetics™ rat and mouse embryonic neuronal cells proliferate?

No, the neurons do not proliferate.
Missing: stable lines

Does the recommended media for each of the diabetic cells contain glucose?

The recommended media does contain glucose. Please contact Scientific Support for glucose concentrations. 
Missing: stable lines

What is the concentration of sugar in Lonza’s endothelial cell media?

While the complete formulation of all of Lonza’s specialty media are proprietary, the EBM™, EBM™-2, and EBM™ phenol red free basal media will all contain D-glucose (dextrose) [CAS number 50-99-7] at exactly 1.0 g/L (5.55 mM). There are no other...
Missing: stable lines
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