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What is the principle behind the ViaLight™ Plus Cell Proliferation and Cytotoxicity Assay?  

ViaLight™ Plus BioAssay Kits use bioluminescence catalysed by the firefly luciferase enzyme to measure intracellular ATP.  Since ATP is central to the metabolism of all cells, the intracellular concentration is very precisely...
Missing: stable linesGuideline

Why do I need to store cell culture media in the dark?

Most tissue culturists will never observe effects on proliferating cells that can be attributed to light exposed media. However, several light-related factors may exert sufficient collective influence to cause the spurious results occasionally...
Missing: stable linesGuideline

What primary human diseased airway cells does Lonza offer?

Lonza's current portfolio for human diseased airway cells includes:- Bronchial Epithelial Cells- Lung Fibroblasts- Bronchial Smooth Muscle Cells...from asthmatic and COPD donors and if available from donors with Cystic fibrosis.An overview...
Missing: stable linesGuideline

Which promoter can be recommended for transient gene expression in mammalian cells ?

Vectors with a CMV promoter typically work fine for transient protein expression in mammalian cells, like HEK293, CHO or NSO. For insect cells, like Sf9 or Sf21, vectors with insect promoters are required. In HEK293E (EBNA) cells, the use...
Missing: stable linesGuideline

How many passages can I use the Clonetics™ diseased cells?

Proliferative capacity of Lonza's Clonetics™ diseased cells is tested through one or more passages (depending on the cell type) for information only.It is NOT a specification where a minimum number of doublings must be obtained in order to pass...
Missing: stable linesGuideline

What kind of effects could a mycoplasma contamination have on my cells?

Mycoplasma contamination can: > Inhibit cell metabolism > Cause cell death > Induce morphological alterations > Alter DNA, RNA, and protein synthesis > Induce chromosomal aberrations > Cause increased sensitivity to inducers...
Missing: stable linesGuideline

Where are Lonza’s Bronchial/Tracheal Epithelial cells isolated from?

Lonza’s Bronchial/Tracheal Epithelial Cells (both with and without Retinoic Acid; both normal and diseased) are isolated from the epithelial cells that line the airway of the bifurcation of the lungs and small sections of the bronchia and trachea...
Missing: stable linesGuideline

How is the disease state for Lonza’s diseased airway cells determined?

The tissue received for all of Lonza’s diseased airway cells are obtained from donors which were diagnosed by an accredited physician prior to death. Frequently, Lonza will obtain information on medications taken related to the disease which...
Missing: stable linesGuideline

Where are Lonza’s Small Airway Epithelial cells isolated from?

Lonza’s Small Airway Epithelial cells (both normal and diseased) are isolated from the distal portion of the lung in the 1 mm bronchiole area. These cells are isolated from airway tubules 1mm in diameter and smaller. Anything with a diameter greater...
Missing: stable linesGuideline

What is the recommended media for culturing Lonza's bone marrow stromal cells?

The recommended media for culturing Lonza's bone marrow stromal cells is MyeloCult™ H5100 from StemCell Technologies, Inc.
Missing: stable linesGuideline
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