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How often do mycoplasma contaminations occur in cell cultures?

A conservative estimate states that between 15-35% of all continuous cell cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).
Missing: stable lines

Where are Lonza's human Mesenchymal Stem Cells isolated from?

Lonza's human Mesenchymal Stem Cells (hMSCs) PT-2501 are isolated from bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers between the ages of 18 and 45 years old. Please see the linked document for more...
Missing: stable lines

Which cell numbers should I use when running the ToxiLight™ Non-Destructive Cytotoxicity BioAssay

Toxilight works much better at low cell numbers, for adherent cell lines < 10,000 in one well of a 96well plate and < 50,000 for suspension.We would normally seed 10,000 cells/well when using adherent cells to allow enough room in the well...
Missing: stable

Can I plate cells at a higher initial concentration than the Clonetics™ and Poietics™ plating protocol recommends? What is the minimum dilution of DMSO most cells can tolerate?

Lonza's recommended plating density for primary cells is established for two main reasons: To ensure residual DMSO is sufficiently diluted while cells are initially attaching and To allow a maximum number of population doublings per passage...
Missing: stable lines

I've had my Clonetics™ Cells in culture for a few months, they're no longer growing and don't look like healthy cells, what's wrong?

All Clonetics™ Cells are primary cells with a finite life span. Most Clonetics™ Cells are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As cell death begins to occur, morphology and cell performance...
Missing: stable lines

What are differences between the endothelial cell types isolated from different locations in the body?

The group of Chi et al analayzed various endothelial cells. Tissue-specific expression patterns in different tissue microvascular endothelial cells suggest they are distinct differentiated cell types that play roles in the local physiology...
Missing: stable lines

What protocol should I use for Nucleofection® of patient derived blood samples, e.g. leukemia or lymphoma cells?

Unfortunately, we do not have a ready-to-use protocol for Chronic lymphocytic leukemia (CLL) or other blood cell derived cancer cells. The cells often have a chromosomal aberration and show the properties of a cell line. Therefore we suggest...
Missing: stable

What is the difference between Lonza’s various renal cell types?

The primary differences between each of Lonza’s various renal cell types are the isolation location and the markers for which each cell types tests positive.Human Renal Epithelial Cells (HRE; Catalog no. CC-2556): Lonza’s Human Renal Epithelial (HRE...
Missing: stable lines

Do intracellular ATP levels differ between different cell types?

Healthy cells in culture contain maximal amounts of ATP and a wide range of metabolic stimuli do not increase these ATP levels.  Any increase in the ATP concentration of a culture can therefore be associated with an increase in the number...
Missing: stable lines

What primary human diseased airway cells does Lonza offer?

Lonza's current portfolio for human diseased airway cells includes:- Bronchial Epithelial Cells- Lung Fibroblasts- Bronchial Smooth Muscle Cells...from asthmatic and COPD donors and if available from donors with Cystic fibrosis.An overview...
Missing: stable lines
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