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306 results sorted by
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Can hydrocortisone be excluded from the Clonetics® BulletKit® when culturing
cell
s? What is the purpose of hydrocortisone in the culture media?
...the original
cell
isolation. While the hydrocortisone will not complete kill or prevent the growth of the contaminating fibroblasts, it will slow the proliferation down so that the other
cell
line
may proliferate and dominate. Hydrocortisone will also help...
Missing:
stable
I've had my Clonetics™
Cell
s in culture for a few months, they're no longer growing and don't look like healthy
cell
s, what's wrong?
All Clonetics™
Cells
are primary
cells
with a finite life span. Most Clonetics™
Cells
are guaranteed for 10 – 15 additional population doublings, which equates to about 2 – 3 passages. As
cell
death begins to occur, morphology and
cell
performance...
Missing:
stable
lines
How do you calculate doubling time of a
cell
culture?
The doubling time is the number of hours it takes for a
cell
population to double in number. Doubling time is calculated using the following equation:Number of hours from 24 hours after seeding until passage/Population Doublings = Doubling Time...
Missing:
stable
lines
How often do mycoplasma contaminations occur in
cell
cultures?
A conservative estimate states that between 15-35% of all continuous
cell
cultures are contaminated with mycoplasma (Drexler HG, Uphof CC; 2002), some estimates are even higher (up to 80 % in some countries (Koshimizu K, Kotani H; 1981).
Missing:
stable
lines
Where are Lonza's human Mesenchymal Stem
Cell
s isolated from?
Lonza's human Mesenchymal Stem
Cells
(hMSCs) PT-2501 are isolated from bone marrow withdrawn from bilateral punctures of the posterior iliac crests of normal volunteers between the ages of 18 and 45 years old. Please see the linked document for more...
Missing:
stable
lines
Where are Lonza’s HSMM
cell
s isolated from?
Lonza obtains our Human Skeletal Muscle Myoblasts( HSMM), both diseased and normal, from quadriceps or psoas muscle tissue.
Missing:
stable
lines
What are differences between the endothelial
cell
types isolated from different locations in the body?
The group of Chi et al analayzed various endothelial
cells
. Tissue-specific expression patterns in different tissue microvascular endothelial
cells
suggest they are distinct differentiated
cell
types that play roles in the local physiology...
Missing:
stable
lines
What is the difference between Lonza’s various renal
cell
types?
The primary differences between each of Lonza’s various renal
cell
types are the isolation location and the markers for which each
cell
types tests positive.Human Renal Epithelial
Cells
(HRE; Catalog no. CC-2556): Lonza’s Human Renal Epithelial (HRE...
Missing:
stable
lines
Do intra
cell
ular ATP levels differ between different
cell
types?
Healthy
cells
in culture contain maximal amounts of ATP and a wide range of metabolic stimuli do not increase these ATP levels. Any increase in the ATP concentration of a culture can therefore be associated with an increase in the number...
Missing:
stable
lines
What is the principle behind the ViaLight™ Plus
Cell
Proliferation and Cytotoxicity Assay?
ViaLight™ Plus BioAssay Kits use bioluminescence catalysed by the firefly luciferase enzyme to measure intracellular ATP. Since ATP is central to the metabolism of all
cells
, the intracellular concentration is very precisely...
Missing:
stable
lines
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