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What percentage of most microvascular lots are lymphatic derived cells (LEC)? What percentage of most microvascular lots are blood vessel derived cells (BEC)?

Each lot of our Human Microvasculare Endothelial Cells - dermal (CC-2543 and CC-2516) and our Human Microvasculare Endothelial Cells - Lung (CC-2527) is tested for the percentage of LEC (double-positive for CD31+ and Podoplanin) and BEC (positive...
Missing: stable lines

If my cell type of interest is not in the cell list on the 4D Nucleofector® Core Unit, how do I choose the program I want to use?

...type in the program you need.This process will need to be repeated for all cuvettes or strip-wells that require a program not linked to a cell type.To save time with future experiments, you can save this as an experiment or use our Custom Program...
Missing: stable lines

What is the general anatomy of the human prostate? From which portion of the human prostate are Lonza's prostate cells are derived?

...the “worker cells” of the tissue or organ.In regards to the prostate specifically, Lonza obtains our Lonza’s Prostate Epithelial Cells (PrEC) from the glandular epithelium (parenchymal portion) of the prostate.The stromal portion of the prostate, or of any...
Missing: stable lines

What protocol should I use for Nucleofection® of patient derived blood samples, e.g. leukemia or lymphoma cells?

Unfortunately, we do not have a ready-to-use protocol for Chronic lymphocytic leukemia (CLL) or other blood cell derived cancer cells. The cells often have a chromosomal aberration and show the properties of a cell line. Therefore we suggest...
Missing: stable

After Nucleofection®, how do you determine cell viability?

We determine cell viability after Nucleofection® in two ways: 1) FACS determination of viable/dead cells by PROPIDIUM IODIDE STAINING. We normally analyze transfection efficiency in living cells by FACS: We first exclude cellular debris...
Missing: stable lines

How many donors are pooled to create the pooled human cells?

Lonza is offering the following primary cells from single donors and from pooled donors:HUVEC (human umbilical vein endothelial cells)NHEK-neo (human epidermal keratinocytes)HMVEC-dNeo (human microvascular endothelial cells, dermal, neonatal)When...
Missing: stable lines

Do I need a special incubator for cultivation of insect cells ?

Insect cells are cultivated at lower temperatures than E.coli or mammalian cells (S2 cells at 24°C, Sf9 cells at 28°C).Therefore they need a dedicated incubator with appropriate temperature.
Missing: stable lines

What medium do you recommend for the diseased airway cells?

The recommended medium for diseased cells is the same as for normal cells. BEGM™ for Diseased Bronchial Epithelial Cells, some lots are guaranteed in B-ALI™ Media. SmGM™-2 Medium is recommended for Diseased Bronchial Smooth Muscle Cells. FGM™-2...
Missing: stable lines

Are there any effects on the differentiation of neural stem cells following Nucleofection®?

The ability of transfected rat or mouse neural stem cells to be subsequently differentiated into a variety of cell types is well documented in:Olig2 Overexpression Induces the In Vitro Differentiation of Neural Stem Cells into Mature...
Missing: stable lines

How does Lonza test for quality of the diseased airway cells?

Diseased Bronchial Epithelial Cells are characterized by morphology through serial passage and some are tested for differentiation using B-ALI™ Differentiation protocols, these are tested for TEER levels, ciliogenesis, and mucin production. Diseased...
Missing: stable lines
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