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Does the reported Log4j vulnerability threat affect our WinKQCL software (Catalog # 25-611E)?

Thank you for using Lonza Endotoxin Detection Products.Lonza has been providing endotoxin detection products and services since the 1970s, leading the way in innovation of products, methods and software.Our dedicated WinKQCL software is on the market...

Automated production of gene-modified chimeric antigen receptor T cells using the Cocoon Platform

Autologous cell-based therapeutics have gained increasing attention in recent years because of their efficacy at treating diseases with limited therapeutic options. Chimeric antigen receptor (CAR) T-cell therapy has demonstrated clinical success in...

Authors: Trainor N, Purpura KA, Middleton K, Fargo K, Hails L, Vicentini-Hogan M, McRobie C, Daniels R,...

SST Peer Reviewed

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how do I convert from EU/ml (Endotoxin Unit) to ng/ml?

Depending on the source of the endotoxin, the conversion from endotoxin units to nanograms will vary. The FDA initially defined the Endotoxin Unit (EU) as the endotoxin activity of 0.2 ng of Reference Endotoxin Standard, EC-2 or 5 EU/ng. To convert...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay should I use matched reagents, lysate/CSE?

You need to use matched reagents in order to comply with FDA requirements for endotoxin testing. Each Lonza LAL lot is tested for functionality using United States Reference Standard EC-7. We then match this LAL lot to a lot of our Control...

When preparing to run a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection kit how is the potency of CSE determined?

The RSE/CSE procedure used by Lonza to determine CSE potency is found on the Certificate of Analysis for each matched kit. In general, dilutions of endotoxin from multiple vials of CSE are tested unknowns compared to a series of standards produced...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I handle the LAL reagent?

Lonza LAL reagent should be handled gently. After reconstitution, the contents of the vial will easily go into solution by either gently inverting or swirling the vial. Vortexing or vigorously shaking the reconstituted LAL reagent will cause it to...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay how should I prepare my sample for testing?

There are a few ways in which your sample can be prepared before testing it. Most samples only need to be diluted before they are tested with one of our endotoxin detection kits. In order to determine how far out you should dilute your sample, you...

When performing a Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor C (rFC), endotoxin detection assay what pH should my sample be? What should I adjust it with?

Our endotoxin detection assays are optimized to work with samples with a pH range of 6-8. If the pH of your sample falls outside of this range you can adjust it with a buffer, or HCl or NaOH. Make sure the buffer you are using is endotoxin free by...

When storing my Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection kit the temperature of my refrigerator/freezer has been out of range. Are my reagents ok to use?

The normal storage temperature for LAL products is 2-8°C. The improper storage of the product for a short period of time should not cause a problem with the performance of the kit as long as the components have not been reconstituted. Lyophilized...

What are the advantages of the Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ Recombinant Factor (rFC), endotoxin detection assays over the rabbit test?

Lonza LAL and PyroGene™ rFC Endotoxin detection assays are faster, have greater sensitivity, show less variability,, are less expensive, and are an alternative to animal model testing. These assays allow higher throughput and can also be automated on...

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