HL-1

HL-1™ is a Serum-free culture medium containing less than 30 µg protein per ml. Components of HL-1 include known amounts of insulin, a variety of saturated and unsaturated fatty acids and proprietary stabilizing bovine proteins. It contains no bovine serum albumin and does not contain L-glutamine.
HL-1™ will support the serum-free growth of various hybridomas and certain other differentiated cells of lymphoid origin. 

Storage = 2°C to 8°C

Shelf Life:
24 months
Recommended for Cells:
CHO-K1 ; Namalwa ; MCF7 ; BT549 ; MCF10A ; THP-1 ; U-937 ; MDCK ; Vero ; P815 ; T cell, human peripheral blood unstim. ; Monocyte, human ; PBMC, human ; Raji ; EL4 ; MOLT-4 ; MDA-MB-231 ; Wehi-3B ; Mononuclear, peripheral blood, human
Categories:
Serum-free and Speciality Media
Research Areas:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Basic Research
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Citations (13)

IL-7 receptor influences anti-TNFresponsiveness and T cell gut homing ininflammatory bowel disease
Categories:
Serum-free and Speciality Media 
Authors:
Lyssia Belarif, Bernard Vanhove, Nicolas Poirier 
In:
J Clin Invest (2019) 129: 5 
Mincle Activation and the Syk/Card9 Signaling Axis are Central to the Development of the Autoimmune Disease of the Eye
Categories:
Serum-free and Speciality Media 
Authors:
Ellen J. Lee, Brieanna R. Brown, Emily E. Vance, Paige E. Snow, Phyllis B. Silver, David Heinrichs, Xin Lin, Yoichiro Iwakura, Christine A. Wells, Rachel R. Caspi and Holly L. Rosenzweig 
In:
J Immunol (2016) 196: 7 
Engineered regulatory T cells coexpressing MHC class II:peptide complexes are efficient inhibitors of autoimmune T cell function and prevent the development of autoimmune arthritis.
Categories:
Serum-free and Speciality Media 
Authors:
Qian Z1, Latham KA, Whittington KB, Miller DC, Brand DD, Rosloniec EF. 
In:
J Immunol (2013) 190(11): 5382-91 
Cutting edge: Leptin-induced ROR?t expression in CD4+ T cells promotes Th17 responses in systemic lupus erythematosus.
Categories:
Serum-free and Speciality Media 
Authors:
Yu Y1, Liu Y, Shi FD, Zou H, Matarese G, La Cava A. 
In:
J Immunol (2013) 190(7): 3054-8 
Activated invariant NKT cells control central nervous system autoimmunity in a mechanism that involves myeloid-derived suppressor cells.
Categories:
Serum-free and Speciality Media 
Authors:
Parekh VV1, Wu L, Olivares-Villagómez D, Wilson KT, Van Kaer L. 
In:
J Immunol (2013) 190(5): 1948-60 
Cutaneous tumors cease CXCL9/Mig production as a result of IFN-?-mediated immunoediting.
Categories:
Serum-free and Speciality Media 
Authors:
Petro M1, Kish D, Guryanova OA, Ilyinskaya G, Kondratova A, Fairchild RL, Gorbachev AV. 
In:
J Immunol (2013) 190(2): 832-41 
TSG-6 protein is crucial for the development of pulmonary hyaluronan deposition, eosinophilia, and airway hyperresponsiveness in a murine model of asthma.
Categories:
 
Authors:
Swaidani S1, Cheng G, Lauer ME, Sharma M, Mikecz K, Hascall VC, Aronica MA. 
In:
J Biol Chem (2013) 288(1): 412-22 
Neutrophil expression of Fas ligand and perforin directs effector CD8 T cell infiltration into antigen-challenged skin.
Categories:
 
Authors:
Kish DD1, Gorbachev AV, Parameswaran N, Gupta N, Fairchild RL. 
In:
J Immunol (2012) 189(5): 2191-202 
SLAT/Def6 plays a critical role in the pathogenic process of experimental autoimmune uveitis (EAU).
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Vistica BP, Shi G, Nugent L, Tan C, Altman A, Gery I. 
In:
Mol Vis (2012) 18: 1858-1864 
Complement protein C1q directs macrophage polarization and limits inflammasome activity during the uptake of apoptotic cells.
Categories:
 
Authors:
Benoit ME1, Clarke EV, Morgado P, Fraser DA, Tenner AJ. 
In:
J Immunol (2012) 188(11): 5682-93 
Blockade of Notch ligand d1 promotes allograft survival by inhibiting alloreactive Th1 cells and cytotoxic T cell generation.
Categories:
 
Authors:
Riella LV1, Ueno T, Batal I, De Serres SA, Bassil R, Elyaman W, Yagita H, Medina-Pestana JO, Chandraker A, Najafian N. 
In:
J Immunol (2011) 187(9): 4629-38 
Identification of a Potential Biomarker for FABP4Inhibition: The Power of Lipidomics in Preclinical Drug Testing
Categories:
Serum-free and Speciality Media 
Authors:
Karsten Suhre, Werner Römisch-Margl, Martin Hrabé de Angelis,Jerzy Adamski, Gerd Luippold, and Robert Augustin 
In:
J Biomol Screen (2011) 16: 5 
Proteoglycan-Induced Arthritis and Recombinant Human Proteoglycan Aggrecan G1 Domain–Induced Arthritis in BALB/c Mice Resembling Two Subtypes of Rheumatoid Arthritis
Categories:
Serum-free and Speciality Media 
Authors:
Tibor T. Glant, Marianna Radacs, Gyorgy Nagyeri, Katalin Olasz, Anna Laszlo, Ferenc Boldizsar, Akos Hegyi, Alison Finnegan, and Katalin Mikecz  
In:
Rheumatology (2011) 63: 5 
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