RPMI-1640

RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures, including fresh human lymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay.

There are a variety of formulations:
12-702         - with L-glutamine
BE12-702/U1 - with UltraGlutamineI
BE15-702D    - [powder] with L-glutamine
12-167          - without L-glutamine
12-115          - with L-glutamine and 25 mM HEPES buffer
BE12-115/U1 - with UltraGlutamineI and 25 mM HEPES buffer
04-525          - with L-glutamine and 165 nM MOPS (used for some mycological assays)
09-774          - with L-glutamine, 25 mM HEPES buffer 100 units/ml penicillin, 50 ug/ml streptomycin
12-918          - without L-glutamine or phenol red
BE12-752       - with L-glutamine, without D-glucose

Storage = 2ºC to 8ºC 
  (versions without L-glutamine and those with UltraGlutamine can be stored at 15ºC to 30ºC)

Shelf Life:
24 months
Categories:
Classical Media and Reagents
Research Areas:
Cancer Research/Cell Biology
Cardiovascular
Dermatology/Tissue Engineering
Immunotherapy / Hematology
Basic Research

Citations (61)

Categories:
Primary Cells and Media, Transfection 
Authors:
Naeimi Kararoudi, M., Dolatshad, H., Trikha, P., Hussain, S. R., Elmas, E., Foltz, J. A., Moseman, J. E., Thakkar, A., Nakkula, R. J., Lamb, M., Chakravarti, N., McLaughlin, K. J., Lee, D. A. 
In:
Journal of Visualized Experiments, JoVE (2018) 136: e58237 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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In:
Scientific Reports (2017) 7: doi: 10.1038/srep43199 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha , Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. 
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Nat Genet (2017) 49(2): 193-203 
Categories:
Live Cell Imaging, Transfection 
Authors:
Yingxin Ma, Mingxiu Wang, Wei Li, Zhiping Zhang, Xiaowei Zhang, Tianwei Tan, Xian-En Zhang, Zongqiang Cui  
In:
Nat Commun. (2017) 15318:  
Categories:
Transfection 
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Cell Rep (2016) 17(5): 1438-1452 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Scientific Reports (2016) 1(6): ePub 
Categories:
Transfection 
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PLoS ONE (2016) 11(2): 1-28 
Categories:
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Scientific Reports (2015) 5:18454: 10.1038 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
Powell JD, Hutchison JR, Hess BM, Straub TM 
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Other (2015) 119(3): 711-23 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media, Transfection 
Authors:
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PLoS ONE (2015) 10(9: e0136644 
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Primary Cells and Media, Classical Media and Reagents 
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Transfection 
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Cancer Immunol Immunother (2015) 3(4): 333-44 
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Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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PLoS ONE (2015) 10(2): e0115736 
Categories:
Primary Cells and Media 
Authors:
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J Biol Chem (2014) 289(51): 35326-40 
Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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J Immunol (2014) 139(9): 4739-47 
Categories:
Primary Cells and Media 
Authors:
Mandl M, Schmitz S, Weber C, Hristov M 
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PLoS ONE (2014) 9(11): ePub 
Categories:
Transfection 
Authors:
Kai X, Chellappa V, Donado C, Reyon D, Sekigami Y, Ataca D, Louissaint A, Mattoo H, Joung JK, Pillai S. 
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J Biol Chem (2014) 289(39): 26960-72 
Categories:
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Authors:
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Categories:
Transfection 
Authors:
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Nucleic Acids Res (2014) 42: 1-16 
Categories:
Transfection 
Authors:
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J Immunol (2014) 192(9): 4043-53 
Categories:
Primary Cells and Media 
Authors:
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Categories:
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Clin Cancer Res (2014) 20(8): 2092-103 
Categories:
Transfection 
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mBio (2014) 5(3): 1-11 
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Classical Media and Reagents, Serum-free and Speciality Media 
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PLoS ONE (2014) 9(3): e93494 
Categories:
Primary Cells and Media 
Authors:
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Blood (2014) 123(12): 1897-904 
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Primary Cells and Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media 
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Categories:
Transfection 
Authors:
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Genome Res (2013) 23(7): 1182-93 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Primary Cells and Media 
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Transfection 
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Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media 
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Categories:
Transfection 
Authors:
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Blood (2011) 118 (7): 1797-1800 
Categories:
Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
Authors:
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Primary Cells and Media, Classical Media and Reagents 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Transfection 
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Categories:
Primary Cells and Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media 
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Categories:
Primary Cells and Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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Classical Media and Reagents, Serum-free and Speciality Media 
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Categories:
Primary Cells and Media, Classical Media and Reagents 
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Primary Cells and Media, Classical Media and Reagents, Serum-free and Speciality Media 
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