Grace's Insect Medium was developed in 1962 and has been shown, with the addition of serum, to support the growth of insect cells, including Spodoptera frugiperda. Unsupplemented Grace's Insect Culture Medium is ideal for transfection because it does not contain lactalbumin hydrolysate, or yeastolate which can interfere with transfection.
When supplemented with L-glutamine, lactalbumin hydrolysate, and yeastolate Grace's Insect Medium can be used for general growth and maintenance of insect cells.

Storage = 2ºC to 8ºC

04-457 has no lactalbumin hydrolysate, yeastolate, gentamycin or FBS
04-649 has lactalbumin hydrolysate, yeastolate, and gentamycin, but no FBS 
04-501 Grace's Complete Insect Medium - has lactalbumin hydrolysate, yeastolate, gentamycin and 10% heat-inactivated FBS