While both hydrocortisone-21-hemisuccinate and ß-glycerophosphate are necessary when differentiating Lonza’s Normal Human Osteoblasts (NHOst), Lonza has noted that some lots of NHOst may be more sensitive to the concentration of hydrocortisone-21-hemisuccinate and/or ß-glycerophosphate. While high concentrations of these reagents may cause greater differentiation in some lots, the same concentrations may cause cells to slough and die in other lots.
???????As such, Lonza suggests trying varying concentrations to find the optimal concentrations for differentiation without sloughing.