Q:

What media do you recommend to grow Bovine Brain Microvascular Endothelial Cells Catalog Number AC-2509 ?

A:

Lonza's EVMB Brain MV Bullet kit AC-3103 is discontinued, but the basal media EBM™-2 is still available.
Add the listed supplements below at the concentrations provided to one 500 ml bottle of EBM™-2 CC-3156 to complete the growth media. All components should be added to one 500 ml bottle of EBM™-2 CC-3156:

  • Beta Endothelial Cell Growth Factor: This component is dissolved in HBSS at a concentration of 1.0 ug/ml (sigma catalog number E1388, CAS # 105843-51-4) 0.5 ml of this component is added to the basal media for a final concentration of 1.0 ng/ml.
  • Heparin: This component is dissolved in HBSS at a concentration of 50 mg/ml. 1.0 ml of this component is added to the basal media for a final concentration of 100 ug/ml.
  • Asorbic Acid Phosphate: This component is dissolved in WFI at a 75 mg/ml. 0.5 ml of this component is added to the basal media for a final concentration of 75 ug/ml.
  • Horse Serum: 50 ml of horse serum should be added to the basal media for a 10% concentration of horse serum.
  • Penicillin/Streptomycin: 0.5 ml of 10 kUnits/ml Penicillin and 10mg/ml Streptomycin should be added to the basal media for a final concentration of 100 units per ml of Penicillin and 101 ug/ml of Streptomycin
Categories:
Primary Cells and Media