Q:

When preforming Lonza Bacterial Endotoxin Test (BET), LAL or PyroGene™ recombinant Factor C (rFC), endotoxin detection kit to establish the potency of Endotoxin Challenge Vials (ECV) can I use a syringe to reconstitute the ECV and other assay reagents?
A:

We do not recommend that a needle and syringe be used to reconstitute ECV or other LAL reagents. The plungers of plastic syringes are generally coated with a lubricant and tend to interfere with the assay. We recommend the tops be removed and diluent added with a pipette.
Categories:
Endotoxin Detection
Research Areas:
Endotoxin Testing

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