Q:

Does it matter if the PBS used for monocyte enrichment contains calcium and magnesium, if the cells should be used later in Nucleofection™ ?

A:

The PBS should be calcium and magnesium free to prevent clumping of cells. We routinely use PBS without calcium and magnesium for example Lonza Cat. No. 17-516F

Categories:
Transfection
Research Areas:
Cancer Research/Cell Biology
Immunotherapy / Hematology
Basic Research