Q:

I stained my gel with SYBR Green and recovered my band of interest. However, my DNA is still green. Can I remove the SYBR stain?

A:

Yes. The SYBR stains can easily be removed from nucleic acids by ethanol precipitation.

Isopropyl alcohol precipitation is somewhat less effective at removing the dye.

Butyl alcohol extraction, chloroform extraction and phenol extraction do not remove the dye efficiently.

Categories:
Electrophoreses and Analysis
Research Areas:
Molecular Biology