Q:

Can I use larger cuvettes for my Nucleofection™ Reaction ? Can I use the cuvettes more than once?

A:

No. The electrical parameters provided by the Nucleofector™ Device are optimized for the cuvettes contained in the Nucleofector™ Kits. The cuvettes are single-use only. Using the cuvettes more than once will result in higher cell death and lower transfection efficiency. Furthermore, cross-contamination e.g. with residual DNA may occur, since Nucleofection™ is a very efficient delivery method.

Categories:
Transfection