Q:

I have been having trouble with "clumping" with my PC12 cells. Do you have any recommendations?

A:

Yes. As PC12 cells often tend to clump we recommend resuspending these cells as thoroughly as possible with a sterile 1mL pipette tip or transfer pipette from your Nucleofectorâ„¢ kit. This circumvents potential fusion of cells due to the electroporation process. Also, switching to a different clone may help as some clones are more prone to clumping than others. For more information, please contact Scientific Support.

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Categories:
Transfection