Can I use Nucleofection™ to create a stable knockdown in a cancer cell line?

Yes. There are several publications available showing stable transfection of an shRNA vector: Cai S et al. (2006) Nat Genet 38:1278-88 (D10.G4.1 cells) Guo F et al. (2005) Cancer Res 65:10536-44 (K562) Hideshima T (2006) Blood 107: 4053-62 (U937) Ng (2006) Cancer Res 66(20): 9903-12 For generation of stable cell lines, please refer to our Technial Reference Guide by clicking on the associated file "Guideline for Generation of Stable Cell Lines ".
Related Cells:
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Research Areas:
Cancer Research/Cell Biology