Q:

DNA-purity and Nucleofection®: Can low A260:A280 ratios lead to both reduced transfection efficiency and cell viability?
A:

Yes. To check the quality of your DNA we strongly recommend determining the A260:A280 ratio.

It should be ideally 1.8 - 2.0 for a good DNA preparation, but least 1.6.
Categories:
Transfection
Research Areas:
Gene Expression

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