MACS™ Selection Kits (Miltenyi Biotec GmbH) offer the opportunity to purify specific blood/immune cell populations by positive selection via antibody-binding or by negative selection using depletion. Which MACS purification method is preferred by Lonza?

We recommend using negative selection (depletion) because your purified cells are â??untouchedâ?, not influenced, and strictly not activated. Positive selection may cause increasing amounts of dead cells and could also lead to activation of the cells due to binding to a specific ligand. Please click on the file "Hematopoetic Cell Purification Method.pdf" to see the methods that were tested by Lonza.
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Research Areas:
Immunotherapy / Hematology