faq

Q:	Do you have data about the in vivo implantation of hepatocytes transfected by Nucleofection™? For how long could protein expression be detected?
A:	There is a publication reporting on the successful reinjection of modified hepatocytes into mice. Chen et al. were able to monitor the expression over several days. This group worked with a preliminary test solution for mouse hepatocyte as the optimized protocol was not finished at this time. Here are the citation details: Plasmid-electroporated primary hepatocytes acquire quasi-physiological secretion of human insulin and restore euglycemia in diabetic mice Authors: Chen NK, Sivalingam J, Tan SY and Kon OL In: Gene Ther (2005) 12(8): 655-667

Do you have data about the in vivo implantation of hepatocytes transfected by Nucleofection™? For how long could protein expression be detected?

There is a publication reporting on the successful reinjection of modified hepatocytes into mice.
Chen et al. were able to monitor the expression over several days. This group worked with a preliminary test solution for mouse hepatocyte as the optimized protocol was not finished at this time. Here are the citation details:

Plasmid-electroporated primary hepatocytes acquire quasi-physiological secretion of human insulin and restore euglycemia in diabetic mice Authors: Chen NK, Sivalingam J, Tan SY and Kon OL In: Gene Ther (2005) 12(8): 655-667

Categories:

Primary Cells and Media

Transfection

Research Areas:

Toxicology