Q:

Do you have a protocol for Nucleofection™ of parasites?

A:

Yes. You can use our Basic Parasite Nucleofector™ Starter kit for parasitic protozoa. The Basic Parasite Nucleofector Starter Kit (Cat.No. VMI-1001) should help you to determine the optimal program and Nucleofector Solution for your parasite-of-interest before using either Basic Parasite Nucleofector Kit 1 or 2. Due to the great number of parasitic protozoa, transfection results can vary significantly between different species and stages. The Nucleofector Technology has been proven to provide high transfection efficiencies in parasitic species such as Plasmodium berghei, Plasmodium yoelii, Trypanosoma brucei, Toxoplasma gondii, Eimeria tenella, Leishmania and Perkinsus marinus. Please note that this kit is not suitable for use with Plasmodium falciparum. For an updated list of successfully transfected parasites, please check our comprehensive cell database.

Related Literature:
Categories:
Transfection
Research Areas:
Parasitology