Our data indicate that the reagents should be placed on the deck prior to running the automated assay. This would be similar to the methodology when performing the assay on the bench. Customers should follow procedures similar to those in a manual assay, specifically, they would not allow reagents to sit on a benchtop for a long period of time prior to use. For the lysate specifically, it is not reconstituted until just prior to transfer to the microplate, so for any time spent on the instrument prior to that, the lysate is in powder form.